Product Info Summary
SKU: | PA1695-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-Tenascin-R TNR Antibody
View all Tenascin R Antibodies
SKU/Catalog Number
PA1695-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Tenascin-R TNR Antibody catalog # PA1695-1. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Tenascin-R TNR Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1695-1)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of mouse TNR, identical to the related rat sequence, and different from the related human sequence by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1695-1 is reactive to Tnr in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
150 kDa
Calculated molecular weight
149562 MW
Background of Tenascin R
Tenascin-R is a protein that in humans is encoded by the TNR gene. Tenascin-R (TNR) is an extracellular matrix protein expressed primarily in the central nervous system. It is a member of the tenascin (TN) gene family, which includes at least 3 genes in mammals: TNC (or hexabrachion), TNX (TNXB), and TNR. The genes are expressed in distinct tissues at different times during embryonic development and are present in adult tissues. TNR has been detected predominantly in the central nervous system and is localized around motor neurons and on motor axons in the spinal cord, cerebellum, hippocampus, and olfactory bulb. It is suggested that tenascin-R has a role in initiating the detachment of neuroblasts from tangential chains and in initiating radial migration of the cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1695-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, Human, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: Rat Brain Tissue, U87 Whole Cell, HELA Whole Cell, MCF-7 Whole Cell
IHC: Rat Brain Tissue
Validation Images & Assay Conditions
Click image to see more details
Anti-TNR antibody, PA1695-1, IHC(P)
IHC(P): Rat Brain Tissue
Click image to see more details
Anti-TNR antibody, PA1695-1, Western blotting
All lanes: Anti TNR (PA1695-1) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: U87 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 150KD
Observed bind size: 150KD
Protein Target Info & Infographic
Gene/Protein Information For Tnr (Source: Uniprot.org, NCBI)
Gene Name
Tnr
Full Name
Tenascin-R
Weight
149562 MW
Superfamily
tenascin family
Alternative Names
Tenascin-R;TN-R;Janusin;Restrictin;TNR; TNR TN-R tenascin R tenascin-R|janusin|restrictin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Tnr, check out the Tnr Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Tnr: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Tenascin-R TNR Antibody (PA1695-1)
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Customer Q&As
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6 Customer Q&As for Anti-Tenascin-R TNR Antibody
Question
Is this PA1695-1 anti-TNR antibody reactive to the isotypes of TNR?
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
The immunogen of PA1695-1 anti-TNR antibody is A synthetic peptide corresponding to a sequence at the N-terminus of mouse TNR(104-117aa QTSDHESQVTFTHK), identical to the related rat sequence, and different from the related human sequence by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-03
Question
We are interested in to test anti-TNR antibody PA1695-1 on mouse amygdala for research purposes, then I may be interested in using anti-TNR antibody PA1695-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
The products we sell, including anti-TNR antibody PA1695-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-29
Question
We are currently using anti-TNR antibody PA1695-1 for mouse tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-13
Answer
The anti-TNR antibody (PA1695-1) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-13
Question
Is a blocking peptide available for product anti-TNR antibody (PA1695-1)?
Verified Customer
Verified customer
Asked: 2019-10-14
Answer
We do provide the blocking peptide for product anti-TNR antibody (PA1695-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-10-14
Question
Will PA1695-1 anti-TNR antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-28
Answer
You can see on the product datasheet, PA1695-1 anti-TNR antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-28
Question
I see that the anti-TNR antibody PA1695-1 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-03-26
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-03-26