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Product Info Summary
| SKU: | A30486 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-TRA-2 alpha Antibody
SKU/Catalog Number
A30486
Size
100ul
Form
Liquid
Description
Boster Bio Anti-TRA-2 alpha Antibody (Catalog# A30486). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TRA-2 alpha Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30486)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human TRA-2 alpha. Immunogen sequence location: 221-270
Reactive Species
A30486 is reactive to TRA2A in Human, Mouse
Observed Molecular Weight
72 kDa
Calculated molecular weight
32.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30486 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ICC/IF 1:200 - 1:1000.
ELISA: 1:20000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western Blot analysis of various cells using Tra-2α Polyclonal Antibody diluted at 1:500. Secondary antibody was diluted at 1:20000.
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Immunofluorescence analysis of HeLa cells, using TRA-2 alpha Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using TRA-2 alpha Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from HUVEC cells, using TRA-2 alpha Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-TRA-2 alpha Antibody (A30486)
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2 Customer Q&As for Anti-TRA-2 alpha Antibody
Question
We are currently using anti-TRA-2 alpha antibody A30486 for mouse tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on primate tissues as well?
V. Evans
Verified customer
Asked: 2019-02-07
Answer
The anti-TRA-2 alpha antibody (A30486) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-02-07
Question
Is this A30486 anti-TRA-2 alpha antibody reactive to the isotypes of TRA2A?
Verified Customer
Verified customer
Asked: 2017-10-24
Answer
The immunogen of A30486 anti-TRA-2 alpha antibody is A peptide derived from human TRA-2 alpha. Immunogen sequence location: 221-270. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-10-24
