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Product Info Summary
| SKU: | M01738 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-Trefoil Factor 3 Monoclonal Antibody
SKU/Catalog Number
M01738
BM5331 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Trefoil Factor 3 Monoclonal Antibody catalog # M01738. Tested in WB, IHC, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Trefoil Factor 3 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01738)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ACHI-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Trefoil Factor 3 Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen) .
Reactive Species
M01738 is reactive to TFF3 in Human
Observed Molecular Weight
13 kDa
Calculated molecular weight
10.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01738 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
IP 1:30
Positive Control
WB: human MCF-7 whole cell
Validation Images & Assay Conditions
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Western blot analysis of Trefoil factor 3/TFF3 using anti-Trefoil factor 3/TFF3 antibody (M01738).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Trefoil factor 3/TFF3 antigen affinity purified monoclonal antibody (M01738) at 1: 500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Trefoil factor 3/TFF3 at approximately 13 kDa. The expected band size for Trefoil factor 3/TFF3 is at 10 kDa.
Specific Publications For Anti-Trefoil Factor 3 Monoclonal Antibody (M01738)
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6 Customer Q&As for Anti-Trefoil Factor 3 Monoclonal Antibody
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for heart using anti-Trefoil Factor 3 Monoclonal antibody M01738. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-25
Question
What sample was tested in WB and IHC applications with M01738?
Verified customer
Asked: 2020-01-07
Answer
Western blot analysis of Trefoil Factor 3 expression in human small intestine lysate.
Boster Scientific Support
Answered: 2020-01-14
Question
We need to test anti-Trefoil Factor 3 Monoclonal antibody M01738 on human heart for research purposes, then I may be interested in using anti-Trefoil Factor 3 Monoclonal antibody M01738 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
B. Miller
Verified customer
Asked: 2017-10-10
Answer
The products we sell, including anti-Trefoil Factor 3 Monoclonal antibody M01738, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-10-10
Question
Do you have a BSA free version of anti-Trefoil Factor 3 Monoclonal antibody M01738 available?
R. Wu
Verified customer
Asked: 2015-12-31
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Trefoil Factor 3 Monoclonal antibody M01738 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-12-31
Question
Does M01738 anti-Trefoil Factor 3 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Z. Bhatt
Verified customer
Asked: 2015-08-25
Answer
It shows on the product datasheet, M01738 anti-Trefoil Factor 3 Monoclonal antibody as been tested on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-08-25
Question
Does anti-Trefoil Factor 3 Monoclonal antibody M01738 work on pig IP with heart?
L. Kulkarni
Verified customer
Asked: 2015-08-18
Answer
Our lab technicians have not tested anti-Trefoil Factor 3 Monoclonal antibody M01738 on pig. You can run a BLAST between pig and the immunogen sequence of anti-Trefoil Factor 3 Monoclonal antibody M01738 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig heart in IP, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-08-18
