Anti-TREX1 Antibody

Product Name

Anti-TREX1 Antibody

View all TREX1 Antibodies

SKU/Catalog Number

A00940

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-TREX1 Antibody (Catalog # A00940). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

TREX1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-TREX1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00940)

Host

Rabbit

Contents

TREX1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

TREX1 antibody was raised against a 15 amino acid synthetic peptide near the center of human TREX1. The immunogen is located within amino acids 220 - 270 of TREX1.

Cross-reactivity

TREX1 antibody will not cross-react with the related protein TREX2. At least three isoforms of TREX1 are known to exist; this antibody will recognize all three isoforms.

Reactive Species

A00940 is reactive to TREX1 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

33.2 kDa

Background of TREX1

Trex1 is the major human 3' to 5' exonuclease which is required for checkpoint signaling after DNA damage. It is ubiquitously expressed, binds to single stranded DNA coated with replication protein A that accumulates at sites of DNA damage and recruits the ataxia telangiectasia and Rad3 related protein (ATR), a checkpoint kinase, to sites of DNA damage and replication stress. Trex1 is required for ATR expression. This gene uses two different open reading frames. The upstream ORF encodes proteins which interact with ATR and localize to intranuclear foci induced by DNA damage and are essential components of the DNA damage checkpoint. The downstream ORF encodes proteins with 3' to 5' exonuclease activity and may be a subunit of human DNA polymerase III. Mutations in this gene result in Aicardi-Goutieres syndrome, chilblain lupus, and Cree encephalitis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00940 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

TREX1 antibody can be used for detection of TREX1 by Western blot at 0.5 and 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information TREX1

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