Anti-TRPV4 Antibody

Product Name

Anti-TRPV4 Antibody

View all TRPV4 Antibodies

SKU/Catalog Number

A00565

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-TRPV4 Antibody (Catalog # A00565). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.

Storage & Handling

TRPV4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-TRPV4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00565)

Host

Rabbit

Contents

TRPV4 antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

Clone: SK7

Isotype

IgG

Immunogen

TRPV4 antibody was raised against an 18 amino acid peptide near the center of human TRPV4. The immunogen is located within amino acids 380 - 430 of TRPV4.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

TRPV4 antibody is human specific. At least six isoforms of TRPV4 are known to exist. This antibody is predicted to not cross-react with other TRP protein family members.

Reactive Species

A00565 is reactive to TRPV4 in Human

Applications

A00565 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

98.281kDa

Background of TRPV4

The transient receptor potential (TRP) protein family consists of a diverse group of cation channels functioning in a variety of homeostatic and regulatory pathways. Four subfamilies exist, based on channel domain homology: C type (canonical), V type (vanilloid receptor related), M type (melastatin related) and P type (PKD) (1). TRPV4, belongs to the V type subfamily and plays a role in systemic osmoregulation (2,3). TRPV4 is a calcium channel multi-pass membrane protein activated by various stimuli, including thermal stress, fatty acid metabolites and hypotonicity (3). TRPV4 is highly expressed in lung and kidney and widely expressed in brain. It plays an important role in regulating neural excitability (4).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

TRPV4 antibody can be used for detection of TRPV4 by Western blot at 1 μg/ml. Antibody can also be used for Immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of TRPV4 in (A) human testis tissue and (B) SK-N-SH cell lysate with TRPV4 antibody at 1 μg/mL.

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Immunohistochemistry of TRPV4 in human lung tissue with TRPV4 antibody at 5 μg/mL.

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Immunofluorescence of TRPV4 in human lung tissue with TRPV4 antibody at 20 μg/mL.

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Immunofluorescence of TRPV4 in human kidney tissue with TRPV4 antibody at 5 μg/ml.

Red: TRPV4 Antibody (A00565)
Blue: DAPI staining

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Immunohistochemistry of TRPV4 in human kidney tissue with TRPV4 antibody at 2 μg/ml.

Gene/Protein Information For TRPV4 (Source: Uniprot.org, NCBI)

Alternative Names

CMT2Cosmosensitive transient receptor potential channel 4; HMSN2C; Osm-9-like TRP channel 4; OTRPC4SSQTL1; transient receptor potential cation channel subfamily V member 4; transient receptor potential cation channel, subfamily V, member 4; Transient receptor potential protein 12; TRP12OSM9-like transient receptor potential channel 4; TrpV4; Vanilloid receptor-like channel 2; Vanilloid receptor-like protein 2; vanilloid receptor-related osmotically activated channel; Vanilloid receptor-related osmotically-activated channel; VRL2; VRL-2; VR-OAC; VROACSPSMA TRPV4 BCYM3, CMT2C, HMSN2C, OTRPC4, SMAL, SPSMA, SSQTL1, TRP12, VRL2, VROAC transient receptor potential cation channel subfamily V member 4 transient receptor potential cation channel subfamily V member 4|OSM9-like transient receptor potential channel 4|osm-9-like TRP channel 4|osmosensitive transient receptor potential channel 4|transient receptor potential protein 12|vanilloid receptor-like channel 2|vanilloid receptor-related osmotically activated channel

For more info on TRPV4, check out the TRPV4 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TRPV4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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