Click image to see more details
Product Info Summary
| SKU: | M01219 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-TRX1 TXN Rabbit Monoclonal Antibody
SKU/Catalog Number
M01219
BM4948 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-TRX1 TXN Rabbit Monoclonal Antibody catalog # M01219. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TRX1 TXN Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01219)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AOGC-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human TRX1
Reactive Species
M01219 is reactive to TXN in Human
Observed Molecular Weight
12 kDa
Calculated molecular weight
11.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01219 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
Positive Control
WB: HepG2 cell
Validation Images & Assay Conditions
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Western blot analysis of TRX1 expression in HepG2 cell lysate.
Specific Publications For Anti-TRX1 TXN Rabbit Monoclonal Antibody (M01219)
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Customer Q&As
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6 Customer Q&As for Anti-TRX1 TXN Rabbit Monoclonal Antibody
Question
Will anti-TRX1 Rabbit Monoclonal antibody M01219 work on pig IF with brain?
Verified Customer
Verified customer
Asked: 2019-08-01
Answer
Our lab technicians have not validated anti-TRX1 Rabbit Monoclonal antibody M01219 on pig. You can run a BLAST between pig and the immunogen sequence of anti-TRX1 Rabbit Monoclonal antibody M01219 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig brain in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-01
Question
Will M01219 anti-TRX1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
You can see on the product datasheet, M01219 anti-TRX1 Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-31
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix using anti-TRX1 Rabbit Monoclonal antibody M01219. Let me know if you need anything else.
Z. Roberts
Verified customer
Asked: 2018-10-22
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-10-22
Question
Is this M01219 anti-TRX1 Rabbit Monoclonal antibody reactive to the isotypes of TXN?
J. Taylor
Verified customer
Asked: 2018-09-20
Answer
The immunogen of M01219 anti-TRX1 Rabbit Monoclonal antibody is A synthesized peptide derived from human TRX1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-09-20
Question
Would anti-TRX1 Rabbit Monoclonal antibody M01219 work for WB with cervix?
W. Lewis
Verified customer
Asked: 2016-09-28
Answer
According to the expression profile of cervix, TXN is highly expressed in cervix. So, it is likely that anti-TRX1 Rabbit Monoclonal antibody M01219 will work for WB with cervix.
Boster Scientific Support
Answered: 2016-09-28
Question
I was wanting to use your anti-TRX1 Rabbit Monoclonal antibody for WB for human cervix on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cervix identification?
H. Taylor
Verified customer
Asked: 2013-01-02
Answer
As indicated on the product datasheet, M01219 anti-TRX1 Rabbit Monoclonal antibody has been validated for IP, IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human cervix in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-01-02
