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Product Info Summary
| SKU: | A05397 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Tubulin beta-1 chain TUBB1 Antibody
SKU/Catalog Number
A05397
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Tubulin beta-1 chain TUBB1 Antibody catalog # A05397. Tested in WB applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Tubulin beta-1 chain TUBB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05397)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human CNG-1beta
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05397 is reactive to TUBB1 in Human, Mouse, Rat
Applications
A05397 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
111 kDa
Calculated molecular weight
50.327kDa
Background of TUBB1
Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis.
Beebe S.J., Mol. Endocrinol. 4:465-475(1990).
Gregory S.G., Nature 441:315-321(2006).
Wu K.-J., Oncogene 21:7872-7882(2002).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
Validation Images & Assay Conditions
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Figure 1. Western blotting validation for Anti-Tubulin beta-1 chain TUBB1 Antibody A05397
Western blot (WB) analysis of TUBB1 polyclonal antibody at 1:500 dilutionLane1:HEK293Twhole cell lysate
Lane2:Raw264.7 whole cell lysate
Lane3:PC12 whole cell lysate
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For TUBB1 (Source: Uniprot.org, NCBI)
Gene Name
TUBB1
Full Name
Tubulin beta-1 chain
Weight
50.327kDa
Superfamily
tubulin family
Alternative Names
beta tubulin 1, class VI; betaI Tubulin; beta-I Tubulin; dJ543J19.4; TUBB1; tubulin beta-1 chain; tubulin, beta 1 TUBB1 tubulin beta 1 class VI tubulin beta-1 chain|tubulin, beta 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TUBB1, check out the TUBB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TUBB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Tubulin beta-1 chain TUBB1 Antibody (A05397)
Hello CJ!
A05397 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Xu T,Song Q,Zhou L,Yang W,Wu X,Qian Q,Chai H,Han Q,Pan H,Dou X,Li S.Ferulic acid alleviates lipotoxicity-induced hepatocellular death through the SIRT1-regulated autophagy pathway and independently of AMPK and Akt in AML-12 hepatocytes.Nutr Metab (Lond).2
Species: Mouse
A05397 usage in article: APP:WB, SAMPLE:AML-12 CELL, DILUTION:NA
Xu T,Song Q,Zhou L,Yang W,Wu X,Qian Q,Chai H,Han Q,Pan H,Dou X,Li S. Ferulic acid alleviates lipotoxicity-induced hepatocellular death through the SIRT1-regulated autophagy pathway and independently of AMPK and Akt in AML-12 hepatocytes. Nutr Metab (Lond)
Species: Mouse
A05397 usage in article: APP:WB, SAMPLE:AML-12 CELLS, DILUTION:NA
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Customer Reviews
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