Anti-TXNDC17 Antibody Picoband™

Product Name

Anti-TXNDC17 Antibody Picoband™

View all Thioredoxin-like 5/TRP14/TXNDC17 Antibodies

SKU/Catalog Number

A09772-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-TXNDC17 Antibody Picoband™ catalog # A09772-1. Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-TXNDC17 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09772-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human TXNDC17 recombinant protein (Position: M1-D123).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A09772-1 is reactive to Txndc17 in Human, Mouse, Rat

Applications

A09772-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

14 kDa

Calculated molecular weight

14.015kDa

Background of Thioredoxin-like 5/TRP14/TXNDC17

Disulfide reductase. May participate in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyze dithiol-disulfide exchange reactions. Modulates TNF-alpha signaling and NF-kappa-B activation. Has peroxidase activity and may contribute to the elimination of cellular hydrogen peroxide.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 µg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of TXNDC17 using anti-TXNDC17 antibody (A09772-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TXNDC17 antigen affinity purified polyclonal antibody (Catalog # A09772-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TXNDC17 at approximately 14 kDa. The expected band size for TXNDC17 is at 14 kDa.

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Figure 2. Flow Cytometry analysis of JK cells using anti-TXNDC17 antibody (A09772-1).
Overlay histogram showing JK cells stained with A09772-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TXNDC17 Antibody (A09772-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For Txndc17 (Source: Uniprot.org, NCBI)

Alternative Names

MGC14353; Protein 42-9-9; thioredoxin (Trx)-related protein, 14 kDa; thioredoxin domain containing 17; thioredoxin domain-containing protein 17; Thioredoxin like 5; Thioredoxin-like 5; Thioredoxin-like protein 5; TRP14; TRP1414 kDa thioredoxin-related protein; TXNDC17; TXNL5 Txndc17|4831443O22Rik, D11Ertd672, D11Ertd672e, TRP, TRP14, Txn, Txnl5|thioredoxin domain containing 17|thioredoxin domain-containing protein 17|14 kDa thioredoxin-related protein|protein 42-9-9|putative 42-9-9 protein|thioredoxin-like 5|thioredoxin-like protein 5

For more info on Txndc17, check out the Txndc17 Infographic

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In this infographic, you will see the following information for Txndc17: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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