Anti-TYW1 Antibody

Product Name

Anti-TYW1 Antibody

View all TYW1 Antibodies

SKU/Catalog Number

A13510

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-TYW1 Antibody (Catalog # A13510). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Rat.

Storage & Handling

TYW1 antibody can be stored at 4 °C, stable for one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-TYW1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13510)

Host

Rabbit

Contents

TYW1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

Clone: SK7

Isotype

IgG

Immunogen

TYW1 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human TYW1. The immunogen is located within amino acids 520 - 570 of TYW1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

TYW1 antibody is predicted to not cross-react with other TYW protein family members. At least three isoforms of TYW1 are known to exist; this antibody will only detect the two larger isoforms.

Reactive Species

A13510 is reactive to TYW1 in Human, Rat

Applications

A13510 is guaranteed for ELISA, IHC-P, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

83.702kDa

Background of TYW1

TYW1, also known as TYW1A, RSAFD1 or YPL207W, is a 732 amino acid protein containing one flavodoxin-like domain that participates in the wybutosine-tRNA (Phe) biosynthesis pathway. Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TYW1 is involved in a multistep enzymatic reaction that stabilizes codon-anticodon base-pairing during the ribosomal decoding process, thereby ensuring correct translation. TYW1 binds to one 4Fe-4S cluster and is located on human chromosome 7. Defects in some of the genes on chromosome 7 have been linked to Osteogenesis imperfecta, Williams-Beuren syndrome, Lissencephaly, Citrullinemia and Shwachman-Diamond syndrome, suggesting that TYW1 may play a role in these conditions

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

TYW1 antibody can be used for detection of TYW1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of TYW1 in human lung tissue lysate with TYW1 antibody at 1 μg/mL.

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Immunohistochemistry of TYW1 in human lung tissue with TYW1 antibody at 5 μg/mL.

Gene/Protein Information For TYW1 (Source: Uniprot.org, NCBI)

Alternative Names

FLJ10900; MGC23001; MGC60291; Radical S-adenosyl methionine and flavodoxin domain-containing protein 1; radical S-adenosyl methionine and flavodoxin domains 1; RSAFD1; tRNA wybutosine-synthesizing protein 1 homolog; tRNA-yW synthesizing protein 1 homolog (S. cerevisiae); tRNA-yW synthesizing protein 1 homolog A; tRNA-yW-synthesizing protein; TYW1A; YPL207W TYW1 RSAFD1A, YPL207W, TYW1 tRNA-yW synthesizing protein 1 homolog S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1|radical S-adenosyl methionine and flavodoxin domain-containing protein 1|radical S-adenosyl methionine and flavodoxin domains 1|tRNA wybutosine-synthesizing protein 1 homolog|tRNA-yW synthesizing protein 1 homolog A

For more info on TYW1, check out the TYW1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TYW1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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