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Product Info Summary
| SKU: | M01491 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-UBE2C Antibody (C-term)
SKU/Catalog Number
M01491
Size
50 µl
Description
Boster Bio Anti-UBE2C Antibody (C-term) (Catalog # M01491). Tested in WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-UBE2C Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01491)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
2001CT211.37.61
Isotype
IgG1,κ
Immunogen
This UBE2C antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 137~172 amino acids from the C-terminal region of human UBE2C.
Reactive Species
M01491 is reactive to UBE2C in Human
Calculated molecular weight
19.7 kDa
Background of UBE2C
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01491 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
Validation Images & Assay Conditions
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All lanes : Anti-UBE2C Antibody (C-term) at 1:2000 dilution
Lane 1: 293T/17 whole cell lysate
Lane 2: A431 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 20 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-UBE2C Antibody (C-term) (M01491)
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