Product Info Summary
SKU: | M02462-1 |
---|---|
Size: | 100 μl/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Ube2N / Ubc13 Rabbit Monoclonal Antibody
View all UBE2N/Ubc13 Antibodies
SKU/Catalog Number
M02462-1
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-Ube2N / Ubc13 Rabbit Monoclonal Antibody catalog # M02462-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ube2N / Ubc13 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02462-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
19U87
Isotype
IgG
Immunogen
A synthesized peptide derived from human Ube2N / Ubc13
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Reactive Species
M02462-1 is reactive to UBE2N in Human, Mouse, Rat
Applications
M02462-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
17 kDa
Calculated molecular weight
17.138kDa
Background of UBE2N/Ubc13
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Ube2N / Ubc13 expression in Daudi cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For UBE2N (Source: Uniprot.org, NCBI)
Gene Name
UBE2N
Full Name
Ubiquitin-conjugating enzyme E2 N
Weight
17.138kDa
Superfamily
ubiquitin-conjugating enzyme family
Alternative Names
bendless-like ubiquitin conjugating enzyme; Bendless-like ubiquitin-conjugating enzyme; BLU; EC 6.3.2.19; MGC131857; MGC8489; UBC13; UbcH13; UbcH-ben; UBE2N; Ubiquitin carrier protein N; ubiquitin-conjugating enzyme E2 N; ubiquitin-conjugating enzyme E2N (homologous to yeast UBC13); ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast); Ubiquitin-protein ligase N; yeast UBC13 homolog UBE2N HEL-S-71, UBC13, UBCHBEN; UBC13, UbcH-ben, UbcH13 ubiquitin conjugating enzyme E2 N ubiquitin-conjugating enzyme E2 N|E2 ubiquitin-conjugating enzyme N|bendless-like ubiquitin conjugating enzyme|epididymis secretory protein Li 71|ubiquitin carrier protein N|ubiquitin conjugating enzyme E2N|ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast)|ubiquitin-conjugating enzyme E2N (homologous to yeast UBC13)|ubiquitin-protein ligase N|yeast UBC13 homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on UBE2N, check out the UBE2N Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for UBE2N: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ube2N / Ubc13 Rabbit Monoclonal Antibody (M02462-1)
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No publications found for M02462-1
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