Anti-UBF1 UBTF Antibody

Product Name

Anti-UBF1 UBTF Antibody

View all UBTF Antibodies

SKU/Catalog Number

A05134

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-UBF1 UBTF Antibody (Catalog # A05134). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-UBF1 UBTF Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05134)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human UBF1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A05134 is reactive to UBTF in Human, Mouse, Rat

Applications

A05134 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

111 kDa

Calculated molecular weight

89.406kDa

Background of UBTF

Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element.

Jantzen H.M., Nature 344:830-836(1990).
Chan E.K.L., J. Exp. Med. 174:1239-1244(1991).
Molina H., Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

Validation Images & Assay Conditions

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Western blot analysis of extracts from HeLa cells and Jurkat cells, using UBF1 antibody A05134.

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Immunohistochemistry analysis of paraffin-embedded human brain tissue using UBF1 antibody A05134.

Gene/Protein Information For UBTF (Source: Uniprot.org, NCBI)

Alternative Names

90-KDa Nucleolus Organizer Region Autoantigen; Autoantigen NOR-90; NOR-90; nucleolar transcription factor 1; UBF; UBF1; UBF-1; UBF2; UBFUBF-1; upstream binding transcription factor, RNA polymerase I; Upstream-binding factor 1 UBTF CONDBA, NOR-90, UBF, UBF-1, UBF1, UBF2 upstream binding transcription factor nucleolar transcription factor 1|90-kDa nucleolus organizer region autoantigen|autoantigen NOR-90|upstream binding transcription factor, RNA polymerase I

For more info on UBTF, check out the UBTF Infographic

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In this infographic, you will see the following information for UBTF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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