Anti-Ubiquitin Antibody (N-term)

Product Name

Anti-Ubiquitin Antibody (N-term)

View all Ubiquitin C Antibodies

SKU/Catalog Number

A00060

Size

80 µl

Form

Liquid

Description

Boster Bio Anti-Ubiquitin Antibody (N-term) (Catalog # A00060). Tested in IHC-P-Leica, WB application(s). This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-Ubiquitin Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00060)

Host

Rabbit

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

This Ubiquitin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-32 amino acids from the N-terminal region of human Ubiquitin.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A00060 is reactive to UBC in Human, Mouse, Rat

Applications

A00060 is guaranteed for WB, IHC-P-Leica Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

77.039kDa

Background of Ubiquitin C

This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:2000
IHC-P-Leica: 1:500

Validation Images & Assay Conditions

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All lanes : Anti-Ubiquitin Antibody (N-term) at 1:2000 dilution
Lane 1: 293 whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: HepG2 whole cell lysate
Lane 4: A549 whole cell lysate
Lane 5: NIH/3T3 whole cell lysate
Lane 6: C2C12 whole cell lysate
Lane 7: C6 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 10 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

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Immunohistochemical analysis of paraffin-embedded Human kidney tissue using A00060 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

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Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue using A00060 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Gene/Protein Information For UBC (Source: Uniprot.org, NCBI)

Alternative Names

ubiquitin C UBC HMG20 ubiquitin C polyubiquitin-C

For more info on UBC, check out the UBC Infographic

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