Anti-Ubiquitin C-terminal Hydrolase 1 (UCHL1) Monoclonal Antibody

Boster Bio Anti-Ubiquitin C-terminal Hydrolase 1 (UCHL1) Monoclonal Antibody catalog # M01018-3. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Bovine, Equine, Human, Mouse, Pig, Rat.

Product Info Summary

SKU: M01018-3
Size: 100ul
Reactive Species: Bovine, Equine, Human, Mouse, Pig, Rat
Host: Mouse
Application: IF, IHC, ICC, WB

Product Name

Anti-Ubiquitin C-terminal Hydrolase 1 (UCHL1) Monoclonal Antibody

See all UCH-L1/PGP9.5 products

SKU/Catalog Number

M01018-3

Size

100ul

Description

Boster Bio Anti-Ubiquitin C-terminal Hydrolase 1 (UCHL1) Monoclonal Antibody catalog # M01018-3. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Bovine, Equine, Human, Mouse, Pig, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Ubiquitin C-terminal Hydrolase 1 (UCHL1) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01018-3)

Host

Mouse

Contents

Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3

Clonality

Monoclonal

Immunogen

Recombinant full length human UCHL1 purified from E. coli.

Reactive Species

M01018-3 is reactive to UCHL1 in Bovine, Equine, Human, Mouse, Pig, Rat

Applications

M01018-3 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot: 1:20,000. ICC/IF and IHC: 1:2,000.

Validation Images & Assay Conditions

Gene/Protein Information For UCHL1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P09936

Gene ID

7345

Gene Name

UCHL1

Full Name

Ubiquitin carboxyl-terminal hydrolase isozyme L1

Weight

24.824kDa

Superfamily

peptidase C12 family

Alternative Names

EC 3.4.19.12; EC 6.-; Neuron cytoplasmic protein 9.5; PARK5; PGP 9.5; PGP9.5; PGP9.5Uch-L1; PGP95; ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase); ubiquitin carboxyl-terminal hydrolase isozyme L1; ubiquitin C-terminal hydrolase; Ubiquitin thioesterase L1; UCHL1; UCH-L1 UCHL1 HEL-117, HEL-S-53, NDGOA, PARK5, PGP 9.5, PGP9.5, PGP95, SPG79, Uch-L1 ubiquitin C-terminal hydrolase L1 ubiquitin carboxyl-terminal hydrolase isozyme L1|epididymis luminal protein 117|epididymis secretory protein Li 53|neuron cytoplasmic protein 9.5|ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)|ubiquitin thioesterase L1|ubiquitin thiolesterase

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on UCHL1, check out the UCHL1 Infographic

UCHL1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for UCHL1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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16 Customer Q&As for Anti-Ubiquitin C-terminal Hydrolase 1 (UCHL1) Monoclonal Antibody

Question

See attached the WB image, lot number and protocol we used for brain using anti-Ubiquitin C-terminal Hydrolase 1 (UCHL1) Monoclonal antibody M01018-3. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-04-08

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-04-08

Question

Is there a BSA free version of anti-Ubiquitin C-terminal Hydrolase 1 (UCHL1) Monoclonal antibody M01018-3 available?

Verified Customer

Verified customer

Asked: 2020-04-07

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Ubiquitin C-terminal Hydrolase 1 (UCHL1) Monoclonal antibody M01018-3 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-04-07

Question

We are currently using anti-Ubiquitin C-terminal Hydrolase 1 (UCHL1) Monoclonal antibody M01018-3 for rat tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says bovine, equine, human, mouse, pig, rat. Is it possible that the antibody can work on pig tissues as well?

R. Zhao

Verified customer

Asked: 2020-03-18

Answer

The anti-Ubiquitin C-terminal Hydrolase 1 (UCHL1) Monoclonal antibody (M01018-3) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-03-18

Question

I see that the anti-Ubiquitin C-terminal Hydrolase 1 (UCHL1) Monoclonal antibody M01018-3 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-11-26

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-11-26

Question

My colleagues were happy with the WB result of your anti-Ubiquitin C-terminal Hydrolase 1 (UCHL1) Monoclonal antibody. However we have seen positive staining in cajal-retzius cell fetal brain cortex cytoplasm. using this antibody. Is that expected? Could you tell me where is UCHL1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-10-31

Answer

Based on literature, cajal-retzius cell fetal brain cortex does express UCHL1. Generally UCHL1 expresses in cytoplasm. Regarding which tissues have UCHL1 expression, here are a few articles citing expression in various tissues:
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 2947814
Lung, and Muscle, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-10-31

Question

Will M01018-3 anti-Ubiquitin C-terminal Hydrolase 1 (UCHL1) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-10-14

Answer

It shows on the product datasheet, M01018-3 anti-Ubiquitin C-terminal Hydrolase 1 (UCHL1) Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-10-14

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-Ubiquitin C-terminal Hydrolase 1 (UCHL1) Monoclonal antibody M01018-3. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-08-26

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-08-26

Question

I was wanting to use your anti-Ubiquitin C-terminal Hydrolase 1 (UCHL1) Monoclonal antibody for WB for pig brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for pig brain identification?

Verified Customer

Verified customer

Asked: 2019-07-15

Answer

It shows on the product datasheet, M01018-3 anti-Ubiquitin C-terminal Hydrolase 1 (UCHL1) Monoclonal antibody has been validated for IHC, WB on bovine, equine, human, mouse, pig, rat tissues. We have an innovator award program that if you test this antibody and show it works in pig brain in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-07-15

Question

Can you help my question with product M01018-3, anti-Ubiquitin C-terminal Hydrolase 1 (UCHL1) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-07-01

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01018-3 anti-Ubiquitin C-terminal Hydrolase 1 (UCHL1) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-07-01

Question

We need to test anti-Ubiquitin C-terminal Hydrolase 1 (UCHL1) Monoclonal antibody M01018-3 on pig brain for research purposes, then I may be interested in using anti-Ubiquitin C-terminal Hydrolase 1 (UCHL1) Monoclonal antibody M01018-3 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-05-03

Answer

The products we sell, including anti-Ubiquitin C-terminal Hydrolase 1 (UCHL1) Monoclonal antibody M01018-3, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-05-03

Question

Is this M01018-3 anti-Ubiquitin C-terminal Hydrolase 1 (UCHL1) Monoclonal antibody reactive to the isotypes of UCHL1?

E. Walker

Verified customer

Asked: 2019-02-04

Answer

The immunogen of M01018-3 anti-Ubiquitin C-terminal Hydrolase 1 (UCHL1) Monoclonal antibody is Recombinant full length human UCHL1 purified from E. coli. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-02-04

Question

We purchased anti-Ubiquitin C-terminal Hydrolase 1 (UCHL1) Monoclonal antibody for IHC on anterior cingulate cortex in the past. I am using equine, and I plan to use the antibody for WB next. you antibody examining anterior cingulate cortex as well as lung muscle in our next experiment. Could give a recommendation on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2018-07-23

Answer

I looked at the website and datasheets of our anti-Ubiquitin C-terminal Hydrolase 1 (UCHL1) Monoclonal antibody and it seems that M01018-3 has been tested on equine in both IHC and WB. Thus M01018-3 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in equine even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-07-23

Question

Will anti-Ubiquitin C-terminal Hydrolase 1 (UCHL1) Monoclonal antibody M01018-3 work for WB with brain?

Verified Customer

Verified customer

Asked: 2018-01-26

Answer

According to the expression profile of brain, UCHL1 is highly expressed in brain. So, it is likely that anti-Ubiquitin C-terminal Hydrolase 1 (UCHL1) Monoclonal antibody M01018-3 will work for WB with brain.

Boster Scientific Support

Answered: 2018-01-26

Question

Is a blocking peptide available for product anti-Ubiquitin C-terminal Hydrolase 1 (UCHL1) Monoclonal antibody (M01018-3)?

W. Wu

Verified customer

Asked: 2017-11-15

Answer

We do provide the blocking peptide for product anti-Ubiquitin C-terminal Hydrolase 1 (UCHL1) Monoclonal antibody (M01018-3). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-11-15

Question

I am interested in using your anti-Ubiquitin C-terminal Hydrolase 1 (UCHL1) Monoclonal antibody for axon target recognition studies. Has this antibody been tested with western blotting on mouse brain? We would like to see some validation images before ordering.

V. Jha

Verified customer

Asked: 2015-03-30

Answer

Thanks for your inquiry. This M01018-3 anti-Ubiquitin C-terminal Hydrolase 1 (UCHL1) Monoclonal antibody is validated on mouse brain, neuronal cells. It is guaranteed to work for IHC, WB in bovine, equine, human, mouse, pig, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2015-03-30

Question

We have seen staining in bovine lung muscle. Any tips? Is anti-Ubiquitin C-terminal Hydrolase 1 (UCHL1) Monoclonal antibody supposed to stain lung muscle positively?

K. Anderson

Verified customer

Asked: 2014-06-24

Answer

From literature lung muscle does express UCHL1. From Uniprot.org, UCHL1 is expressed in anterior cingulate cortex, lung muscle, brain, cajal-retzius cell fetal brain cortex, among other tissues. Regarding which tissues have UCHL1 expression, here are a few articles citing expression in various tissues:
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 2947814
Lung, and Muscle, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2014-06-24

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M01018-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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