Anti-Ubiquitin-like protein ATG12 ATG12 Antibody

Product Name

Anti-Ubiquitin-like protein ATG12 ATG12 Antibody

View all ATG12 Antibodies

SKU/Catalog Number

A00820

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Ubiquitin-like protein ATG12 ATG12 Antibody (Catalog # A00820). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

ATG12 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Ubiquitin-like protein ATG12 ATG12 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00820)

Host

Rabbit

Contents

ATG12 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

ATG12 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human ATG12. The immunogen is located within the first 50 amino acids of ATG12.

Reactive Species

A00820 is reactive to ATG12 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

15.1 kDa

Background of ATG12

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG12, another member of the autophagy protein family, forms a conjugate with ATG5; this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells. ATG12 has also been shown to interact with ATG10 in human embryonic kidney cells in the presence of ATG7. At least two isoforms of ATG12 are known to exist.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00820 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ATG12 antibody can be used for the detection of ATG10 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information ATG12

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