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Product Info Summary
| SKU: | A09782 |
|---|---|
| Size: | 100 µg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-UCP4 SLC25A27 Antibody
SKU/Catalog Number
A09782
Size
100 µg
Form
Liquid
Description
Boster Bio Anti-UCP4 SLC25A27 Antibody (Catalog# A09782). Tested in WB application(s). This antibody reacts with Human.
Storage & Handling
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Cite This Product
Anti-UCP4 SLC25A27 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09782)
Host
Rabbit
Contents
100 μg in 200 μl PBS containing 0.05% BSA and 0.05% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A partial length recombinant UCP4 protein (amino acids 150-320)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A09782 is reactive to SLC25A27 in Human
Applications
A09782 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
36.064kDa
Background of UCP4
SmarcAL1 (SWI/SNF-related µatrix-associated actin-dependent regulator of chromatin a-like 1) is also known as HARP, HepA Related Protein, HHARP, SMARCA like Protein 1 and SWI/SNF Related. SmarcAL1 is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein shows sequence similarity to the E. coli RNA polymerase-binding protein HepA. Mutations in this gene are a cause of Schimke immunoosseous dysplasia (SIOD), an autosomal recessive disorder with the diagnostic features of spondyloepiphyseal dysplasia, renal dysfunction, and T-cell immunodeficiency.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot: 2-4 µg/ml
Validation Images & Assay Conditions
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Western blot analysis of UCP4. Anti-UCP4 antibody was used at 4 &mu
Protein Target Info & Infographic
Gene/Protein Information For SLC25A27 (Source: Uniprot.org, NCBI)
Gene Name
SLC25A27
Full Name
Mitochondrial uncoupling protein 4
Weight
36.064kDa
Superfamily
mitochondrial carrier (TC 2.A.29) family
Alternative Names
FLJ33552; mitochondrial uncoupling protein 4; Solute carrier family 25 member 27; solute carrier family 25, member 27; UCP 4; UCP4uncoupling protein 4 SLC25A27 UCP4 solute carrier family 25 member 27 mitochondrial uncoupling protein 4|UCP 4|uncoupling protein 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SLC25A27, check out the SLC25A27 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SLC25A27: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-UCP4 SLC25A27 Antibody (A09782)
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No publications found for A09782
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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4 Customer Q&As for Anti-UCP4 SLC25A27 Antibody
Question
Will anti-UCP4 antibody A09782 work for WB with esophagus?
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
According to the expression profile of esophagus, SLC25A27 is highly expressed in esophagus. So, it is likely that anti-UCP4 antibody A09782 will work for WB with esophagus.
Boster Scientific Support
Answered: 2020-02-20
Question
I have a question about product A09782, anti-UCP4 antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Z. Lewis
Verified customer
Asked: 2019-11-21
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A09782 anti-UCP4 antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-21
Question
We are currently using anti-UCP4 antibody A09782 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2018-05-16
Answer
The anti-UCP4 antibody (A09782) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-05-16
Question
I see that the anti-UCP4 antibody A09782 works with WB, what is the protocol used to produce the result images on the product page?
N. Williams
Verified customer
Asked: 2015-03-20
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-03-20
