Product Info Summary
SKU: | M00584 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-ULK1/Atg1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00584
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-ULK1/Atg1 Rabbit Monoclonal Antibody catalog # M00584. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ULK1/Atg1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00584)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ABHH-21
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ULK1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00584 is reactive to ULK1 in Human, Mouse, Rat
Applications
M00584 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
130 kDa
Calculated molecular weight
112.631kDa
Background of ULK1
Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of ULK1 using anti-ULK1 antibody (M00584).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human U-87MG whole cell lysates,
Lane 4: human Hela whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ULK1 antigen affinity purified monoclonal antibody (Catalog # M00584) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ULK1 at approximately 130 kDa. The expected band size for ULK1 is at 113 kDa.
Click image to see more details
Figure 2. IHC analysis of ULK1 using anti-ULK1 antibody (M00584).
ULK1 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ULK1 Antibody (M00584) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of ULK1 using anti-ULK1 antibody (M00584).
ULK1 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ULK1 Antibody (M00584) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of ULK1 using anti-ULK1 antibody (M00584).
ULK1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ULK1 Antibody (M00584) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of ULK1 using anti-ULK1 antibody (M00584).
ULK1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ULK1 Antibody (M00584) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Immunofluorescent analysis of 293 cells, using ULK1 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For ULK1 (Source: Uniprot.org, NCBI)
Gene Name
ULK1
Full Name
Serine/threonine-protein kinase ULK1
Weight
112.631kDa
Superfamily
protein kinase superfamily
Alternative Names
ATG1 autophagy related 1 homolog; ATG1; ATG1A; EC 2.7.11; EC 2.7.11.1; FLJ38455; FLJ46475; KIAA0722; serine/threonine-protein kinase ULK1; unc-51 (C. elegans)-like kinase 1; UNC51; Unc51.1; unc-51-like kinase 1 (C. elegans); Unc-51-like kinase 1 ULK1 ATG1, ATG1A, UNC51, Unc51.1, hATG1 unc-51 like autophagy activating kinase 1 serine/threonine-protein kinase ULK1|ATG1 autophagy related 1 homolog|autophagy-related protein 1 homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ULK1, check out the ULK1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ULK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ULK1/Atg1 Rabbit Monoclonal Antibody (M00584)
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No publications found for M00584
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Customer Reviews
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Customer Q&As
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2 Customer Q&As for Anti-ULK1/Atg1 Rabbit Monoclonal Antibody
Question
Could you please provide the immunogen sequence for product M00584?
Verified customer
Asked: 2022-09-12
Answer
The immunogen sequence for M00584 is FLDKQRLLDRIHSITAERL.
Boster Scientific Support
Answered: 2022-09-12
Question
We are currently using anti-ULK1/Atg1 Rabbit Monoclonal antibody M00584 for human tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2018-02-08
Answer
The anti-ULK1/Atg1 Rabbit Monoclonal antibody (M00584) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-08