Anti-UROP11 Apba1 Antibody

Product Name

Anti-UROP11 Apba1 Antibody

View all APBA1 Antibodies

SKU/Catalog Number

A05440

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-UROP11 Apba1 Antibody (Catalog # A05440). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

UROP11 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-UROP11 Apba1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05440)

Host

Rabbit

Contents

UROP11 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

UROP11 antibody was raised against a 12 amino acid synthetic peptide near the center of the mouse UROP11. The immunogen is located within the first 50 amino acids of UROP11.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least four isoforms of GPAT1 are known to exist; this antibody will detect only the two largest isoforms.

Reactive Species

A05440 is reactive to APBA1 in Human, Mouse, Rat

Applications

A05440 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

92.865kDa

Background of APBA1

Upregulated obese product 11 (UROP11) was isolated from subtractive library for mouse hypothalamus. It is detected in numerous tissues and is upregulated in the hypothalamus and adipocytes in obese mice. UROP11 is also observed in human neural and lymphoid tissues; its expression appears to be regulated by the level of lymphoid activation. Urop11 expression is strongly upregulated both in vivo in mouse hypothalamus and in vitro in mouse neural cell lines after treatment with leptin, indicating Urop11 is a target of leptin activity and thus may play a role in obesity.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

UROP11 antibody can be used for detection of UROP11 by Western blot at 1 - 2 μg/mL. Despite its predicted molecular weight, UROP11 often migrates at a much higher apparent size in SDS-PAGE.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of UROP11 in mouse spleen tissue lysate with UROP11 antibody at (A) 1 and (B) 2 μg/mL.

Gene/Protein Information For APBA1 (Source: Uniprot.org, NCBI)

Alternative Names

Adapter protein X11alpha; amyloid beta (A4) precursor protein-binding, family A, member 1; amyloid beta A4 precursor protein-binding family A member 1; D9S411Eadaptor protein X11alpha; LIN10; mint-1; MINT1amyloid beta (A4) precursor protein-binding, family A, member 1 (X11); Neuronal Munc18-1-interacting protein 1; Neuron-specific X11 protein; phosphotyrosine-binding/-interacting domain (PTB)-bearing protein; UROP11; X11A; X11X11ALPHA APBA1 D9S411E, LIN10, MINT1, X11, X11A, X11ALPHA amyloid beta precursor protein binding family A member 1 amyloid-beta A4 precursor protein-binding family A member 1|adapter protein X11alpha|adaptor protein X11alpha|amyloid beta (A4) precursor protein-binding, family A, member 1 (X11)|mint-1|neuron-specific X11 protein|neuronal munc18-1-interacting protein 1|phosphotyrosine-binding/-interacting domain (PTB)-bearing protein

For more info on APBA1, check out the APBA1 Infographic

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In this infographic, you will see the following information for APBA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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