Anti-Uroplakin Ib/UPIb Antibody Picoband™

Product Name

Anti-Uroplakin Ib/UPIb Antibody Picoband™

View all Uroplakin Ib Antibodies

SKU/Catalog Number

A10230-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Uroplakin Ib/UPIb Antibody Picoband™ catalog # A10230-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Uroplakin Ib/UPIb Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10230-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Uroplakin Ib/UPIb recombinant protein (Position: I107-Q216).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

A10230-1 is reactive to UPK1B in Human, Mouse, Rat

Applications

A10230-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

30 kDa

Calculated molecular weight

29.643kDa

Background of Uroplakin Ib

Uroplakin 1B, also known as UPK1B, is a protein which in humans is encoded by the UPK1B gene. The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is found in the asymmetrical unit membrane (AUM) where it can form a complex with other transmembrane 4 superfamily proteins. It may play a role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane through AUM/cytoskeletal interactions. The use of alternate polyadenylation sites has been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Direct ELISA, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Uroplakin Ib/UPIb using anti-Uroplakin Ib/UPIb antibody (A10230-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human SK-OV-3 whole cell lysates,
Lane 3: rat stomach tissue lysates,
Lane 4: mouse stomach tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Uroplakin Ib/UPIb antigen affinity purified polyclonal antibody (Catalog # A10230-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Uroplakin Ib/UPIb at approximately 30KD. The expected band size for Uroplakin Ib/UPIb is at 30KD.

Gene/Protein Information For UPK1B (Source: Uniprot.org, NCBI)

Alternative Names

tetraspanin-20; TSPAN20; tspan-20; TSPAN20tetraspan; UP1b; UPIB; UPK1; UPK1B; Uroplakin 1B; Uroplakin Ib; uroplakin-1b UPK1B TSPAN20, UPIB, UPK1 uroplakin 1B uroplakin-1b|UP1b|tetraspan|tetraspanin-20|tspan-20|uroplakin Ib

For more info on UPK1B, check out the UPK1B Infographic

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In this infographic, you will see the following information for UPK1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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