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Product Info Summary
| SKU: | M00045-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC |
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Product info
Product Name
Anti-VEGF VEGFA Rabbit Monoclonal Antibody
SKU/Catalog Number
M00045-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-VEGF VEGFA Rabbit Monoclonal Antibody catalog # M00045-1. Tested in IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-VEGF VEGFA Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00045-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ADB-22
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human VEGF
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00045-1 is reactive to VEGFA in Human, Mouse
Applications
M00045-1 is guaranteed for Flow Cytometry, IF, IHC, ICC Boster Guarantee
Observed Molecular Weight
83, 88 kDa
Calculated molecular weight
27.042kDa
Background of VEGFA
Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns (4,5) P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) . PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:100-1:250
ICC/IF 1:100-1:250
FC 1:50
Validation Images & Assay Conditions
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunohistochemical analysis of paraffin-embedded human kindey, using VEGF Antibody.
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Immunofluorescent analysis of HUVEC cells, using VEGF Antibody.
Protein Target Info & Infographic
Gene/Protein Information For VEGFA (Source: Uniprot.org, NCBI)
Gene Name
VEGFA
Full Name
Vascular endothelial growth factor A
Weight
27.042kDa
Superfamily
PDGF/VEGF growth factor family
Alternative Names
MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor VEGFA MVCD1, VEGF, VPF vascular endothelial growth factor A vascular endothelial growth factor A|vascular endothelial growth factor A121|vascular endothelial growth factor A165|vascular permeability factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on VEGFA, check out the VEGFA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for VEGFA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-VEGF VEGFA Rabbit Monoclonal Antibody (M00045-1)
Hello CJ!
M00045-1 has been cited in 31 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhou D,Huang X,Xie Y,Deng Z,Guo J,Huang H.Astrocytes-derived VEGF exacerbates the microvascular damage of late delayed RBI.Neuroscience.2019 Jun 1;408:14-21.doi:10.1016/j.neuroscience.2019.03.039.Epub 2019 Mar 22.PMID:30910640.
Species: Mouse
M00045-1 usage in article: APP:WB, SAMPLE:BRAIN TISSUE, DILUTION:1:300
Junyi Tang,Chunyan Liu,Bing Xu,Dawei Wang,Zhenshen Ma,Xiaotian Chang,ARHGEF10L contributes to liver tumorigenesis through RhoA-ROCK1 signaling and the epithelial-mesenchymal transition,Experimental Cell Research,Volume 374,Issue 1,2019,Pages 46-68,ISSN 0014-4827,https://doi.org/10.1016/j.yexcr.2018.11.007.
Species: Human,Mouse
M00045-1 usage in article: APP:WB, SAMPLE:HEPATIC TISSUE, DILUTION:NA
Shao JB,Li Z,Zhang N,Yang F,Gao W,Sun ZG.Hypoxia-inducible factor 1α in combination with vascular endothelial growth factor could predict the prognosis of postoperative patients with oesophageal squamous cell cancer.Pol J Pathol.2019;70(2):84-90.doi:10.5114/pjp.2019. 87100.PMID:31556558.
Species: Human
M00045-1 usage in article: APP:IHC, SAMPLE:OSCC TISSUE, DILUTION:1:100
Hu L,Sun Y,Luo J,He X,Ye M,Li G,Zhang Y,Bai J,Zhang D,Chang C.Targeting TR4 nuclear receptor with antagonist bexarotene increases docetaxel sensitivity to better suppress the metastatic castration-resistant prostate cancer progression.Oncogene.2020 Feb;39
Species: Human
M00045-1 usage in article: APP:IHC, SAMPLE:PCA TISSUE, DILUTION:NA
Telmisartan-enhanced hypercholesterolaemic serum-induced vascular endothelial growth factor expression in immortalized human umbilical vascular endothelial cells
AQP-4 in peritumoral edematous tissue is correlated with the degree of glioma and with expression of VEGF and HIF-alpha.
Deer thymosin beta 10 functions as a novel factor for angiogenesis and chondrogenesis during antler growth and regeneration
Vitamin K2 Ameliorates Damage of Blood Vessels by Glucocorticoid: a Potential Mechanism for Its Protective Effects in Glucocorticoid-induced Osteonecrosis of the Femoral Head in a Rat Model
Effect of CPU-XT-008, a combretastatin A-4 analogue, on the proliferation, apoptosis and expression of vascular endothelial growth factor and basic fibroblast growth factor in human umbilical vein endothelial cells
Arginine ADP-ribosyltransferase 1 promotes angiogenesis in colorectal cancer via the PI3K/Akt pathway
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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17 Customer Q&As for Anti-VEGF VEGFA Rabbit Monoclonal Antibody
Question
We have observed staining in mouse lung. Do you have any suggestions? Is anti-VEGF Rabbit Monoclonal antibody supposed to stain lung positively?
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
From what I have seen in literature lung does express VEGFA. From what I have seen in Uniprot.org, VEGFA is expressed in left lobe of thyroid gland, kidney, mammary gland, renal glomerulus, hemangioendothelioma, heart, lung, retina, among other tissues. Regarding which tissues have VEGFA expression, here are a few articles citing expression in various tissues:
Heart, Pubmed ID: 19054851
Kidney, Pubmed ID: 9878851, 12124351
Lung, Pubmed ID: 15489334
Mammary gland, Pubmed ID: 9450968
Renal glomerulus, Pubmed ID: 10464055
Retina, Pubmed ID: 10067980, 11563986
Boster Scientific Support
Answered: 2020-04-23
Question
I see that the anti-VEGF Rabbit Monoclonal antibody M00045-1 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-04-09
Question
We want using your anti-VEGF Rabbit Monoclonal antibody for positive regulation of p38mapk cascade studies. Has this antibody been tested with western blotting on huvec cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-03-31
Answer
We appreciate your inquiry. This M00045-1 anti-VEGF Rabbit Monoclonal antibody is validated on huvec cells, hela cells. It is guaranteed to work for Flow Cytometry, IP, IF, IHC, ICC in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-03-31
Question
We bought anti-VEGF Rabbit Monoclonal antibody for ICC on left lobe of thyroid gland a few months ago. I am using human, and We intend to use the antibody for IHC next. We need examining left lobe of thyroid gland as well as lung in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
T. Jackson
Verified customer
Asked: 2019-12-31
Answer
I have checked the website and datasheets of our anti-VEGF Rabbit Monoclonal antibody and it appears that M00045-1 has been tested on human in both ICC and IHC. Thus M00045-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-12-31
Question
Will M00045-1 anti-VEGF Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
It shows on the product datasheet, M00045-1 anti-VEGF Rabbit Monoclonal antibody as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-16
Question
I was wanting to use your anti-VEGF Rabbit Monoclonal antibody for Flow Cytometry for mouse heart on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse heart identification?
Verified Customer
Verified customer
Asked: 2019-08-26
Answer
It shows on the product datasheet, M00045-1 anti-VEGF Rabbit Monoclonal antibody has been tested for Flow Cytometry, IP, IF, IHC, ICC on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse heart in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-26
Question
Is a blocking peptide available for product anti-VEGF Rabbit Monoclonal antibody (M00045-1)?
Verified Customer
Verified customer
Asked: 2019-07-16
Answer
We do provide the blocking peptide for product anti-VEGF Rabbit Monoclonal antibody (M00045-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-16
Question
We are currently using anti-VEGF Rabbit Monoclonal antibody M00045-1 for mouse tissue, and we are satisfied with the IP results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
The anti-VEGF Rabbit Monoclonal antibody (M00045-1) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-29
Question
Can you confirm it works in Immunohistochemistry (frozen tissues) on mouse?
Verified customer
Asked: 2019-05-10
Answer
The Anti-VEGF VEGFA Rabbit Monoclonal Antibody (M00045-1) is able to work for IHC-F. However, we haven't performed flow cytometry on mouse samples. There is only an FCM image on human sample: https://www.bosterbio.com/anti-vegf-rabbit-monoclonal-antibody-m00045-1-boster.html?options=cart
Boster Scientific Support
Answered: 2019-05-14
Question
Is there a BSA free version of anti-VEGF Rabbit Monoclonal antibody M00045-1 available?
Verified Customer
Verified customer
Asked: 2019-05-02
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-VEGF Rabbit Monoclonal antibody M00045-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-02
Question
Is this M00045-1 anti-VEGF Rabbit Monoclonal antibody reactive to the isotypes of VEGFA?
N. Walker
Verified customer
Asked: 2019-04-15
Answer
The immunogen of M00045-1 anti-VEGF Rabbit Monoclonal antibody is A synthesized peptide derived from human VEGF. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-04-15
Question
Does anti-VEGF Rabbit Monoclonal antibody M00045-1 work for Flow Cytometry with heart?
Verified Customer
Verified customer
Asked: 2018-12-19
Answer
According to the expression profile of heart, VEGFA is highly expressed in heart. So, it is likely that anti-VEGF Rabbit Monoclonal antibody M00045-1 will work for Flow Cytometry with heart.
Boster Scientific Support
Answered: 2018-12-19
Question
Please see the WB image, lot number and protocol we used for heart using anti-VEGF Rabbit Monoclonal antibody M00045-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-07-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-18
Question
My question regards to test anti-VEGF Rabbit Monoclonal antibody M00045-1 on mouse heart for research purposes, then I may be interested in using anti-VEGF Rabbit Monoclonal antibody M00045-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
D. Roberts
Verified customer
Asked: 2017-12-25
Answer
The products we sell, including anti-VEGF Rabbit Monoclonal antibody M00045-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-12-25
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for heart using anti-VEGF Rabbit Monoclonal antibody M00045-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-09-25
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-09-25
Question
I have a question about product M00045-1, anti-VEGF Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Carter
Verified customer
Asked: 2017-04-06
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00045-1 anti-VEGF Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-04-06
Question
My boss were satisfied with the WB result of your anti-VEGF Rabbit Monoclonal antibody. However we have seen positive staining in left lobe of thyroid gland secreted using this antibody. Is that expected? Could you tell me where is VEGFA supposed to be expressed?
F. Mangal
Verified customer
Asked: 2013-02-05
Answer
From literature, left lobe of thyroid gland does express VEGFA. Generally VEGFA expresses in secreted. Regarding which tissues have VEGFA expression, here are a few articles citing expression in various tissues:
Heart, Pubmed ID: 19054851
Kidney, Pubmed ID: 9878851, 12124351
Lung, Pubmed ID: 15489334
Mammary gland, Pubmed ID: 9450968
Renal glomerulus, Pubmed ID: 10464055
Retina, Pubmed ID: 10067980, 11563986
Boster Scientific Support
Answered: 2013-02-05
