Anti-Vegfc Antibody Picoband™

Product Name

Anti-Vegfc Antibody Picoband™

View all VEGF-C Antibodies

SKU/Catalog Number

A00623-3

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-Vegfc Antibody Picoband™ catalog # A00623-3. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Vegfc Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00623-3)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived mouse Vegfc recombinant protein (Position: A108-R223).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00623-3 is reactive to VEGFC in Mouse, Rat

Applications

A00623-3 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

46 kDa

Calculated molecular weight

46.883kDa

Background of VEGF-C

Vascular endothelial growth factor C (VEGF-C) is a protein that is a member of the platelet-derived growth factor / vascular endothelial growth factor (PDGF/VEGF) family. It is encoded in humans by the VEGFC gene, which is located on chromosome 4q34. Enables vascular endothelial growth factor receptor 3 binding activity. Involved in several processes, including negative regulation of osteoblast differentiation; positive regulation of angiogenesis; and positive regulation of mesenchymal stem cell proliferation. Acts upstream of or within several processes, including cellular response to leukemia inhibitory factor; positive regulation of lymphangiogenesis; and positive regulation of peptidyl-tyrosine phosphorylation. Located in extracellular space. Is expressed in several structures, including alimentary system mesentery; brain; cardiovascular system; embryo mesenchyme; and genitourinary system. Used to study hereditary lymphedema. Human ortholog(s) of this gene implicated in breast carcinoma and hereditary lymphedema ID. Orthologous to human VEGFC (vascular endothelial growth factor C).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Mouse, Rat
ELISA, 0.1-0.5 µg/ml, Mouse

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Vegfc using anti-Vegfc antibody (A00623-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Vegfc antigen affinity purified polyclonal antibody (Catalog # A00623-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Vegfc at approximately 46 kDa. The expected band size for Vegfc is at 46 kDa.

Gene/Protein Information For VEGFC (Source: Uniprot.org, NCBI)

Alternative Names

Flt4 ligand; Flt4-L; vascular endothelial growth factor C; Vascular endothelial growth factor-related protein; VEGFC; VEGF-C; VRPFLT4 ligand DHM VEGFC Flt4-L, LMPH1D, LMPHM4, VRP vascular endothelial growth factor C vascular endothelial growth factor C|FLT4 ligand DHM|vascular endothelial growth factor-related protein

For more info on VEGFC, check out the VEGFC Infographic

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In this infographic, you will see the following information for VEGFC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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