|Validated Species:||Chicken, Human, Mouse, Rat|
Data & Images
|Product Name||Anti-Vinculin Antibody (Monoclonal, hVIN-1)|
|Description||Mouse IgG monoclonal antibody for Vinculin, vinculin (VCL) detection. Tested with WB, IHC-F in Human;mouse;rat;chicken. No cross reactivity with other proteins.|
|Cite This Product||Anti-Vinculin Antibody (Monoclonal, hVIN-1) hVIN-1 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1103)|
|Replacement Item||This antibody may replace the following items: sc-25336|sc-55465|sc-5573|sc-59803|sc-73264|sc-73614|sc-7648|sc-7649 from Santa Cruz Biotechnology.|
|Validated Species||Chicken, Human, Mouse, Rat|
|Predicted Species||Bovine, Rabbit|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||Human vinculin, purified from uterus.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Molecular Weight||116615 MW|
|Protein Function||Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion (By similarity). .|
|Tissue Specificity||VCL: Metavinculin is muscle-specific.|
|Sequence Similarities||Belongs to the vinculin/alpha-catenin family.|
|Subcellular Localization||Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell junction, adherens junction . Cell junction, focal adhesion . Cytoplasm, cytoskeleton . Recruitment to cell-cell junctions occurs in a myosin II-dependent manner. Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions. .|
|Alternative Names||Vinculin ;Metavinculin ;Vcl ;|
|Research Areas|||signal transduction|cytoskeleton / ecm|cytoskeleton|microfilaments|actin etc|actin binding proteins| cardiovascular|heart|cardiac arrhythmias|contractility|contractile proteins|actins| isotype/loading controls|vinculin||
Background for Vinculin
Anti-Vinculin Antibody (Monoclonal, hVIN-1) Images
Click the images to enlarge.
IHC(F): Human Placenta Tissue
IHC(F): Human Placenta Tissue