SKU MA1103
Size 100μg/vial
Reactivity Chicken, Human, Mouse, Rat
Clonality Monoclonal
Clone Number hVIN-1
Host Mouse
Ig Isotype Mouse IgG1
Applications IHC, WB

Overview

Product Name Anti-Vinculin Antibody (Monoclonal, hVIN-1)
SKU/Catalog Number MA1103
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for Vinculin, vinculin (VCL) detection. Tested with WB, IHC-F in Human;mouse;rat;chicken. No cross reactivity with other proteins.
Cite This Product Anti-Vinculin Antibody (Monoclonal, hVIN-1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1103)
Host Mouse
Immunogen Human vinculin, purified from uterus.
Reactivity Chicken, Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Immunohistochemistry(Frozen Section), 2-4μg/ml, Human, chicken, mouse, rat, -
Western blot, 1-2μg/ml, Human, chicken, mouse, rat

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F).

Images And Assay Conditions

/antibody/ma1103 2 IHC anti vinculin antibody monoclonal hvin 1.jpg

Anti- Vinculin antibody, MA1103, IHC(P)
IHC(F): Human Placenta Tissue

/antibody/ma1103 3 IHC anti vinculin antibody monoclonal hvin 1.jpg

Anti- Vinculin antibody, MA1103, IHC(P)
IHC(F): Human Placenta Tissue

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P85972
Gene Name Vcl
Protein Name Vinculin
Tissue Specificity VCL: Metavinculin is muscle-specific.
Alternative Names Vinculin ;Metavinculin ;Vcl ;
Subcellular Localization Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell junction, adherens junction . Cell junction, focal adhesion . Cytoplasm, cytoskeleton . Recruitment to cell-cell junctions occurs in a myosin II-dependent manner. Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions. .
Molecular Weight 116615 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion (By similarity). .
Background Vinculin is a cytoskeletal protein associated with the cytoplasmic face of both cell-cell and cell-extracellular matrix adherens-type junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Both human and chicken embryo sequences of vinculin contain 1,066 amino acids and, furthermore, that the 2 proteins exhibit a high level of sequence identity(greater than 95%). Vinculinis mapped to 10q22.1-q23.

Order Product (MA1103)

Promotion:

Buy primary get secondary antibody for free.
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1050 $280
100ug+Free Biotin Secondary BA1001 $280
Ships in 5-7 business days.

Troubleshooting

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Publications

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Yao Q, An Y, Hou W, Cao YN, Yao MF, Ma NN, Hou L, Zhang H, Liu HJ, Zhang B. Oncotarget. 2017 Nov 30;8(65):109632-109645. doi: 10.18632/oncotarget.22759. eCollection 2017 Dec 12. LRP6 promotes invasion and metastasis of colorectal cancer through cytoskeleton dynamics
Lyu J, Hu Y, Xu X, Zhang H. J Cell Biochem. 2013 Aug;114(8):1744-59. Doi: 10.1002/Jcb.24517. Dynamics Of Focal Adhesions And Reorganization Of F-Actin In Vegf-Stimulated Nscs Under Varying Differentiation States.
Wang H, Wang X, Qu J, Yue Q, Hu Y, Zhang H. J Cell Physiol. 2015 Nov;230(11):2728-42. Doi: 10.1002/Jcp.24997. Vegf Enhances The Migration Of Mscs In Neural Differentiation By Regulating Focal Adhesion Turnover.
Lyu Z, Wang H, Wang Y, Ding K, Liu H, Yuan L, Shi X, Wang M, Wang Y, Chen H. Nanoscale. 2014 Jun 21;6(12):6959-69. Doi: 10.1039/C4Nr01540A. Maintaining The Pluripotency Of Mouse Embryonic Stem Cells On Gold Nanoparticle Layers With Nanoscale But N...

Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.