Anti-Vinculin VCL Antibody (Monoclonal, hVIN-1)

Boster Bio Anti-Vinculin VCL Antibody (Monoclonal, hVIN-1) catalog # MA1103. Tested in IF, IHC-F, ICC, WB applications. This antibody reacts with Chicken, Human, Mouse, Rat. Cited in 10 publication(s).

Product Info Summary

SKU: MA1103
Size: 100μg/vial
Reactive Species: Chicken, Human, Mouse, Rat
Host: Mouse
Application: IF, IHC-F, ICC, WB

Product Name

Anti-Vinculin VCL Antibody (Monoclonal, hVIN-1)

See all Vinculin products

SKU/Catalog Number

MA1103

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Vinculin VCL Antibody (Monoclonal, hVIN-1) catalog # MA1103. Tested in IF, IHC-F, ICC, WB applications. This antibody reacts with Chicken, Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Vinculin VCL Antibody (Monoclonal, hVIN-1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1103)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

hVIN-1

Isotype

Mouse IgG1

Immunogen

Human vinculin, purified from uterus.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

MA1103 is reactive to VCL in Chicken, Human, Mouse, Rat

Applications

MA1103 is guaranteed for IF, IHC-F, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Frozen Section), 2-4μg/ml, Human, chicken, mouse, rat, -
Western blot, 1-2μg/ml, Human, chicken, mouse, rat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For VCL (Source: Uniprot.Org, NCBI)

Uniprot ID

P85972

Gene ID

305679

Gene Name

VCL

Full Name

Vinculin

Weight

116.615kDa

Superfamily

vinculin/alpha-catenin family

Alternative Names

CMD1W; CMH15; metavinculin; MVCL; VCL; Vinculin Vcl|vinculin|vinculin|metavinculin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on VCL, check out the VCL Infographic

VCL infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for VCL: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

MA1103 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Wang K,Xing ZH,Jiang QW,Yang Y,Huang JR,Yuan ML,Wei MN,Li Y,Wang ST,Liu K,Shi Z.Targeting uPAR by CRISPR/Cas9 System Attenuates Cancer Malignancy and Multidrug Resistance.Front Oncol.2019 Feb 27;9:80.doi:10.3389/fonc.2019.00080.PMID:30873379;PMCID:PMC6400983.
Species: Human
MA1103 usage in article: APP:WB, SAMPLE:HCT8/T CELL AND KBV200 CELL, DILUTION:NA

Dong Y,Chen Y,Zhu D,Shi K,Ma C,Zhang W,Rocchi P,Jiang L,Liu X.Self-assembly of amphiphilic phospholipid peptide dendrimer-based nanovectors for effective delivery of siRNA therapeutics in prostate cancer therapy.J Control Release.2020 Jun 10;322:416-425.d
Species: Human,Mouse
MA1103 usage in article: APP:WB, SAMPLE:PC-3 CELL, DILUTION:NA

Targeting ABCB1-mediated tumor multidrug resistance by CRISPR/Cas9-based genome editing Yang Yang,1,et al. Am J Transl Res. 2016; 8(9): 3986–3994. Published online 2016 Sep 15.

Effects of atorvastatin on porcine aqueous humour outflow and trabecular meshwork cells

Yao Q, An Y, Hou W, Cao YN, Yao MF, Ma NN, Hou L, Zhang H, Liu HJ, Zhang B. Oncotarget. 2017 Nov 30;8(65):109632-109645. doi: 10.18632/oncotarget.22759. eCollection 2017 Dec 12. LRP6 promotes invasion and metastasis of colorectal cancer through cy...

Lyu J, Hu Y, Xu X, Zhang H. J Cell Biochem. 2013 Aug;114(8):1744-59. Doi: 10.1002/Jcb.24517. Dynamics Of Focal Adhesions And Reorganization Of F-Actin In Vegf-Stimulated Nscs Under Varying Differentiation States.

Wang H, Wang X, Qu J, Yue Q, Hu Y, Zhang H. J Cell Physiol. 2015 Nov;230(11):2728-42. Doi: 10.1002/Jcp.24997. Vegf Enhances The Migration Of Mscs In Neural Differentiation By Regulating Focal Adhesion Turnover.

Lyu Z, Wang H, Wang Y, Ding K, Liu H, Yuan L, Shi X, Wang M, Wang Y, Chen H. Nanoscale. 2014 Jun 21;6(12):6959-69. Doi: 10.1039/C4Nr01540A. Maintaining The Pluripotency Of Mouse Embryonic Stem Cells On Gold Nanoparticle Layers With Nanoscale But N...

Shen Z, Lu D, Li Q, Zhang Z, Zhu Y. Biomed Res Int. 2015;2015:871202. Doi: 10.1155/2015/871202. Epub 2015 Mar 8. Synthesis And Characterization Of Biodegradable Polyurethane For Hypopharyngeal Tissue Engineering.

Su F,, Liu Ss,, Ma Jl, Wang Ds, E Ll, Liu Hc. Stem Cell Res Ther. 2015 Mar 12;6:22. Doi: 10.1186/S13287-015-0023-3. Enhancement Of Periodontal Tissue Regeneration By Transplantation Of Osteoprotegerin-Engineered Periodontal Ligament Stem Cells.

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16 Customer Q&As for Anti-Vinculin VCL Antibody (Monoclonal, hVIN-1)

Question

Is a blocking peptide available for product anti-Vinculin antibody (Monoclonal, hVIN-1) (MA1103)?

Verified Customer

Verified customer

Asked: 2020-04-03

Answer

We do provide the blocking peptide for product anti-Vinculin antibody (Monoclonal, hVIN-1) (MA1103). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-04-03

Question

See attached the WB image, lot number and protocol we used for prostate using anti-Vinculin antibody (Monoclonal, hVIN-1) MA1103. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-03-24

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-03-24

Question

Is this MA1103 anti-Vinculin antibody (Monoclonal, hVIN-1) reactive to the isotypes of VCL?

Verified Customer

Verified customer

Asked: 2020-02-19

Answer

The immunogen of MA1103 anti-Vinculin antibody (Monoclonal, hVIN-1) is Human vinculin, purified from uterus. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-02-19

Question

We were well pleased with the WB result of your anti-Vinculin antibody (Monoclonal, hVIN-1). However we have seen positive staining in retina cell membrane using this antibody. Is that expected? Could you tell me where is VCL supposed to be expressed?

P. Evans

Verified customer

Asked: 2019-12-03

Answer

According to literature, retina does express VCL. Generally VCL expresses in cell membrane. Regarding which tissues have VCL expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Endothelial cell, Pubmed ID: 2116004
Liver, Pubmed ID: 24275569
Platelet, Pubmed ID: 12665801
Prostate, Pubmed ID: 15489334
Retina, Pubmed ID: 17974005
Uterus, Pubmed ID: 1339348

Boster Scientific Support

Answered: 2019-12-03

Question

Does anti-Vinculin antibody (Monoclonal, hVIN-1) MA1103 work for IHC-F with prostate?

Verified Customer

Verified customer

Asked: 2019-11-28

Answer

According to the expression profile of prostate, VCL is highly expressed in prostate. So, it is likely that anti-Vinculin antibody (Monoclonal, hVIN-1) MA1103 will work for IHC-F with prostate.

Boster Scientific Support

Answered: 2019-11-28

Question

I was wanting to use your anti-Vinculin antibody (Monoclonal, hVIN-1) for IHC-F for human prostate on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human prostate identification?

E. Walker

Verified customer

Asked: 2019-11-14

Answer

You can see on the product datasheet, MA1103 anti-Vinculin antibody (Monoclonal, hVIN-1) has been tested for IF, IHC-F, ICC, WB on chicken, human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human prostate in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-11-14

Question

Would MA1103 anti-Vinculin antibody (Monoclonal, hVIN-1) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-08-01

Answer

You can see on the product datasheet, MA1103 anti-Vinculin antibody (Monoclonal, hVIN-1) as been tested on IHC-F. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-08-01

Question

Do you have a BSA free version of anti-Vinculin antibody (Monoclonal, hVIN-1) MA1103 available?

Verified Customer

Verified customer

Asked: 2019-07-24

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Vinculin antibody (Monoclonal, hVIN-1) MA1103 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-07-24

Question

I see that the anti-Vinculin antibody (Monoclonal, hVIN-1) MA1103 works with IHC-F, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-07-11

Answer

You can find protocols for IHC-F on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-07-11

Question

We purchased anti-Vinculin antibody (Monoclonal, hVIN-1) for WB on liver a few months ago. I am using rat, and We want to use the antibody for ICC next. I am looking for examining liver as well as cervix carcinoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for ICC?

Verified Customer

Verified customer

Asked: 2019-07-01

Answer

I took a look at the website and datasheets of our anti-Vinculin antibody (Monoclonal, hVIN-1) and I see that MA1103 has been tested on rat in both WB and ICC. Thus MA1103 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-07-01

Question

We have seen staining in mouse uterus. Any tips? Is anti-Vinculin antibody (Monoclonal, hVIN-1) supposed to stain uterus positively?

Verified Customer

Verified customer

Asked: 2018-01-25

Answer

From literature uterus does express VCL. From Uniprot.org, VCL is expressed in myometrium, endothelial cell, retina, prostate, fetal brain cortex, uterus, platelet, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have VCL expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Endothelial cell, Pubmed ID: 2116004
Liver, Pubmed ID: 24275569
Platelet, Pubmed ID: 12665801
Prostate, Pubmed ID: 15489334
Retina, Pubmed ID: 17974005
Uterus, Pubmed ID: 1339348

Boster Scientific Support

Answered: 2018-01-25

Question

Can you help my question with product MA1103, anti-Vinculin antibody (Monoclonal, hVIN-1). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2017-12-20

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1103 anti-Vinculin antibody (Monoclonal, hVIN-1), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-12-20

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for prostate using anti-Vinculin antibody (Monoclonal, hVIN-1) MA1103. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2017-10-27

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-10-27

Question

My question regards using your anti-Vinculin antibody (Monoclonal, hVIN-1) for r-rno-6798695; neutrophil degranulation studies. Has this antibody been tested with western blotting on mouse nih3t3 whole cell lysates? We would like to see some validation images before ordering.

P. Brown

Verified customer

Asked: 2017-09-21

Answer

Thanks for your inquiry. This MA1103 anti-Vinculin antibody (Monoclonal, hVIN-1) is validated on human hela, mouse nih3t3 whole cell lysates, hela whole cell lysates, hepg2 whole cell lysates, rat rh35 whole cell lysates. It is guaranteed to work for IF, IHC-F, ICC, WB in chicken, human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2017-09-21

Question

I am looking for to test anti-Vinculin antibody (Monoclonal, hVIN-1) MA1103 on human prostate for research purposes, then I may be interested in using anti-Vinculin antibody (Monoclonal, hVIN-1) MA1103 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

J. Parker

Verified customer

Asked: 2017-05-09

Answer

The products we sell, including anti-Vinculin antibody (Monoclonal, hVIN-1) MA1103, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-05-09

Question

We are currently using anti-Vinculin antibody (Monoclonal, hVIN-1) MA1103 for chicken tissue, and we are content with the IF results. The species of reactivity given in the datasheet says chicken, human, mouse, rat. Is it true that the antibody can work on horse tissues as well?

V. Mangal

Verified customer

Asked: 2017-03-16

Answer

The anti-Vinculin antibody (Monoclonal, hVIN-1) (MA1103) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-03-16

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MA1103
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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