SKU MA1103
Size 100μg/vial
Reactivity Chicken, Human, Mouse, Rat
Clonality Monoclonal
Clone Number hVIN-1
Host Mouse
Ig Isotype Mouse IgG1
Applications IF, IHC-F, ICC, WB

Overview

Product Name Anti-Vinculin Antibody (Monoclonal, hVIN-1)
SKU/Catalog Number MA1103
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for Vinculin, vinculin (VCL) detection. Tested with WB, IHC-F, ICC/IF in Human;mouse;rat;chicken. No cross reactivity with other proteins.
Cite This Product Anti-Vinculin Antibody (Monoclonal, hVIN-1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1103)
Host Mouse
Contents/Buffer Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Form Lyophilized
Immunogen Human vinculin, purified from uterus.
Reactivity Chicken, Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Concentration: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Immunohistochemistry(Frozen Section), 2-4μg/ml, Human, chicken, mouse, rat, -
Western blot, 1-2μg/ml, Human, chicken, mouse, rat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F) and ICC.

Images And Assay Conditions

/antibody/ma1103 2 IHC anti vinculin antibody monoclonal hvin 1.jpg

Figure 1. IHC analysis of Vinculin using anti-Vinculin antibody (MA1103).
Vinculin was detected in frozen section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Vinculin Antibody (MA1103) overnight at 4°C. Biotinylated goat anti- mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

/antibody/ma1103 3 IHC anti vinculin antibody monoclonal hvin 1.jpg

Figure 2. IHC analysis of Vinculin using anti-Vinculin antibody (MA1103).
Vinculin was detected in frozen section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Vinculin Antibody (MA1103) overnight at 4°C. Biotinylated goat anti- mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

/m/a/ma1103 3_1.jpg

Figure 3. Western blot analysis of Vinculin using anti-Vinculin antibody (MA1103).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela, whole cell lysates,
Lane 2: human HepG2, whole cell lysates,
Lane 3: monkey COS-7 whole cell lysates,
Lane 4: human U-87MG whole cell lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: rat RH35 whole cell lysates,
Lane 7: mouse HEPA1-6 whole cell lysates,
Lane 8: mouse NIH3T3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Vinculin antigen affinity purified monoclonal antibody (Catalog # MA1103) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti- mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Vinculin at approximately 124KD. The expected band size for Vinculin is at 124KD.

/m/a/ma1103 4.jpg

Figure 4. IF analysis of Vinculin using anti- Vinculin antibody (MA1103)
Vinculin was detected in immunocytochemical section of human mammary cancer cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL mouse anti- Vinculin Antibody (MA1103) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P85972
Gene Name VCL
Protein Name Vinculin
Tissue Specificity VCL: Metavinculin is muscle-specific.
Alternative Names Vinculin ;Metavinculin ;Vcl ;
Subcellular Localization Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell junction, adherens junction . Cell junction, focal adhesion . Cytoplasm, cytoskeleton . Recruitment to cell-cell junctions occurs in a myosin II-dependent manner. Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions. .
Molecular Weight 116615 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion (By similarity). .
Research Areas Actin Binding Proteins, Actin Etc, Cardiovascular, Contractility, Cytoskeleton, Cytoskeleton / Ecm, Microfilaments, Signal Transduction

*You can search these to find other products in these research areas.
Background Vinculin is a cytoskeletal protein associated with the cytoplasmic face of both cell-cell and cell-extracellular matrix adherens-type junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Both human and chicken embryo sequences of vinculin contain 1,066 amino acids and, furthermore, that the 2 proteins exhibit a high level of sequence identity(greater than 95%). Vinculinis mapped to 10q22.1-q23.

Other Recommended Resources

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Order Product (MA1103)

Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1050 $280
100ug+Free Biotin Secondary BA1001 $280

USD $280

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Publications

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Synthesis and Characterization of Biodegradable Polyurethane for Hypopharyngeal Tissue Engineering
Yao Q, An Y, Hou W, Cao YN, Yao MF, Ma NN, Hou L, Zhang H, Liu HJ, Zhang B. Oncotarget. 2017 Nov 30;8(65):109632-109645. doi: 10.18632/oncotarget.22759. eCollection 2017 Dec 12. LRP6 promotes invasion and metastasis of colorectal cancer through cytoskeleton dynamics
Lyu J, Hu Y, Xu X, Zhang H. J Cell Biochem. 2013 Aug;114(8):1744-59. Doi: 10.1002/Jcb.24517. Dynamics Of Focal Adhesions And Reorganization Of F-Actin In Vegf-Stimulated Nscs Under Varying Differentiation States.
Wang H, Wang X, Qu J, Yue Q, Hu Y, Zhang H. J Cell Physiol. 2015 Nov;230(11):2728-42. Doi: 10.1002/Jcp.24997. Vegf Enhances The Migration Of Mscs In Neural Differentiation By Regulating Focal Adhesion Turnover.
Lyu Z, Wang H, Wang Y, Ding K, Liu H, Yuan L, Shi X, Wang M, Wang Y, Chen H. Nanoscale. 2014 Jun 21;6(12):6959-69. Doi: 10.1039/C4Nr01540A. Maintaining The Pluripotency Of Mouse Embryonic Stem Cells On Gold Nanoparticle Layers With Nanoscale But N...

Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to support@bosterbio.com.