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- Table of Contents
VCL antibody (M01207) performs well in IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 3 Publications
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Immunofluorescent analysis of A673 cells, using Vinculin Antibody .
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Anti-Vinculin VCL Rabbit Monoclonal Antibody
VCL antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IP, IF, IHC, ICC, WB |
Tested Applications
VCL antibody was positively detected by WB in:
human Hela whole cell, human HepG2 whole cell, human U-87MG whole cell, rat PC-12 whole cell, mouse NIH/3T3 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
VCL antibody was positively detected by IHC in:
human colorectal adenocarcinoma tissue, human renal cell carcinoma tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
VCL antibody was positively detected by ICC/IF in:
A673 cell
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
IP
Human
Tested
Tested
Recommend
Recommend
Rat
Tested
Recommend
Mouse
Tested
Recommend
Product Information
Product Name
Anti-Vinculin VCL Rabbit Monoclonal Antibody
View all VCL Antibodies
View all VCL Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AIG-22
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Vinculin
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
124 kDa
Calculated Molecular Weight
123.8 kDa
Cite This Product
Anti-Vinculin VCL Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01207)
Protocols
Western Blot protocol for VCL antibody
Immunohistochemistry (IHC) protocol for VCL antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for VCL antibody
Publications
Application:
Species:
Macrophage-derived exosome as bioengineered nanotherapeutics for silica-induced reproductive dysregulation
Published: 2026-02-14
Journal: JOURNAL OF HAZARDOUS MATERIALS
Application: IF,WB
Impact Factor: 11.30
Species: Mouse
PMID: 41702166
Biodegradable Magnesium alloy Janus membrane with surface-selective osteoinduction and soft tissue healing properties in guided bone regeneration
Published: 2025-02-09
Journal: Acta Biomaterialia
Application: IF
Impact Factor: 9.60
Species: Mouse,Human
PMID: 39933642
Effects of atorvastatin on porcine aqueous humour outflow and trabecular meshwork cells
Published: 2017-10-23
Journal: Experimental and Therapeutic Medicine
Application: WB
Impact Factor: 1.26
Species: Mouse
PMID: 29250149
Review
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Product Promise
We promise Anti-Vinculin VCL Rabbit Monoclonal Antibody performs excellently in relevant applications for IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M01207
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 13, 2026