Anti-Vinculin VCL Rabbit Monoclonal Antibody

Boster Bio Anti-Vinculin VCL Rabbit Monoclonal Antibody catalog # M01207. Tested in IP, IF, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 4 publication(s).

Product Info Summary

SKU: M01207
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IP, IF, WB

Product Name

Anti-Vinculin VCL Rabbit Monoclonal Antibody

See all Vinculin products

SKU/Catalog Number

M01207

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Vinculin VCL Rabbit Monoclonal Antibody catalog # M01207. Tested in IP, IF, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Vinculin VCL Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01207)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AIG-22

Immunogen

A synthesized peptide derived from human Vinculin

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01207 is reactive to VCL in Human, Mouse, Rat

Applications

M01207 is guaranteed for IP, IF, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
ICC/IF 1:50-1:200
IP 1:50

Validation Images & Assay Conditions

Gene/Protein Information For VCL (Source: Uniprot.Org, NCBI)

Uniprot ID

P18206

Gene ID

7414

Gene Name

VCL

Full Name

Vinculin

Weight

123.799kDa

Superfamily

vinculin/alpha-catenin family

Alternative Names

CMD1W; CMH15; metavinculin; MVCL; VCL; Vinculin VCL CMD1W, CMH15, HEL114, MV, MVCL vinculin vinculin|epididymis luminal protein 114|epididymis secretory sperm binding protein|meta-vinculin|metavinculin|vinculin isoform meta-VCL

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on VCL, check out the VCL Infographic

VCL infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for VCL: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

M01207 has been cited in 4 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Wang K,Xing ZH,Jiang QW,Yang Y,Huang JR,Yuan ML,Wei MN,Li Y,Wang ST,Liu K,Shi Z.Targeting uPAR by CRISPR/Cas9 System Attenuates Cancer Malignancy and Multidrug Resistance.Front Oncol.2019 Feb 27;9:80.doi:10.3389/fonc.2019.00080.PMID:30873379;PMCID:PMC6400983.
Species: Human
M01207 usage in article: APP:WB, SAMPLE:HCT8/T CELL AND KBV200 CELL, DILUTION:NA

Yao Q, An Y, Hou W, Cao YN, Yao MF, Ma NN, Hou L, Zhang H, Liu HJ, Zhang B. Oncotarget. 2017 Nov 30;8(65):109632-109645. doi: 10.18632/oncotarget.22759. eCollection 2017 Dec 12. LRP6 promotes invasion and metastasis of colorectal cancer through cy...

Shen Z, Lu D, Li Q, Zhang Z, Zhu Y. Biomed Res Int. 2015;2015:871202. Doi: 10.1155/2015/871202. Epub 2015 Mar 8. Synthesis And Characterization Of Biodegradable Polyurethane For Hypopharyngeal Tissue Engineering.

Su F,, Liu Ss,, Ma Jl, Wang Ds, E Ll, Liu Hc. Stem Cell Res Ther. 2015 Mar 12;6:22. Doi: 10.1186/S13287-015-0023-3. Enhancement Of Periodontal Tissue Regeneration By Transplantation Of Osteoprotegerin-Engineered Periodontal Ligament Stem Cells.

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16 Customer Q&As for Anti-Vinculin VCL Rabbit Monoclonal Antibody

Question

I was wanting to use your anti-Vinculin Rabbit Monoclonal antibody for WB for human uterus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human uterus identification?

Verified Customer

Verified customer

Asked: 2020-03-24

Answer

You can see on the product datasheet, M01207 anti-Vinculin Rabbit Monoclonal antibody has been validated for IP, IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human uterus in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-03-24

Question

Do you have a BSA free version of anti-Vinculin Rabbit Monoclonal antibody M01207 available?

Verified Customer

Verified customer

Asked: 2020-03-13

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Vinculin Rabbit Monoclonal antibody M01207 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-03-13

Question

We purchased anti-Vinculin Rabbit Monoclonal antibody for WB on cervix carcinoma in a previous experiment. I am using mouse, and We are going to use the antibody for IF next. I was wanting to use examining cervix carcinoma as well as uterus in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?

Verified Customer

Verified customer

Asked: 2020-01-03

Answer

I looked at the website and datasheets of our anti-Vinculin Rabbit Monoclonal antibody and I see that M01207 has been tested on mouse in both WB and IF. Thus M01207 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-01-03

Question

Is this M01207 anti-Vinculin Rabbit Monoclonal antibody reactive to the isotypes of VCL?

Verified Customer

Verified customer

Asked: 2019-12-04

Answer

The immunogen of M01207 anti-Vinculin Rabbit Monoclonal antibody is A synthesized peptide derived from human Vinculin. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-12-04

Question

We have been able to see staining in rat liver. What should we do? Is anti-Vinculin Rabbit Monoclonal antibody supposed to stain liver positively?

Verified Customer

Verified customer

Asked: 2019-11-26

Answer

Based on literature liver does express VCL. Based on Uniprot.org, VCL is expressed in myometrium, endothelial cell, retina, prostate, fetal brain cortex, uterus, platelet, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have VCL expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Endothelial cell, Pubmed ID: 2116004
Liver, Pubmed ID: 24275569
Platelet, Pubmed ID: 12665801
Prostate, Pubmed ID: 15489334
Retina, Pubmed ID: 17974005
Uterus, Pubmed ID: 1339348

Boster Scientific Support

Answered: 2019-11-26

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for uterus using anti-Vinculin Rabbit Monoclonal antibody M01207. Let me know if you need anything else.

Z. Singh

Verified customer

Asked: 2019-11-05

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-05

Question

We were well pleased with the WB result of your anti-Vinculin Rabbit Monoclonal antibody. However we have seen positive staining in liver cell membrane using this antibody. Is that expected? Could you tell me where is VCL supposed to be expressed?

D. Jackson

Verified customer

Asked: 2019-07-30

Answer

From literature, liver does express VCL. Generally VCL expresses in cell membrane. Regarding which tissues have VCL expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Endothelial cell, Pubmed ID: 2116004
Liver, Pubmed ID: 24275569
Platelet, Pubmed ID: 12665801
Prostate, Pubmed ID: 15489334
Retina, Pubmed ID: 17974005
Uterus, Pubmed ID: 1339348

Boster Scientific Support

Answered: 2019-07-30

Question

Does M01207 anti-Vinculin Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-07-22

Answer

It shows on the product datasheet, M01207 anti-Vinculin Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-07-22

Question

We are interested in using your anti-Vinculin Rabbit Monoclonal antibody for signaling by high-kinase activity braf mutants studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2018-12-21

Answer

I appreciate your inquiry. This M01207 anti-Vinculin Rabbit Monoclonal antibody is validated on hepg2 cell lysate, hela cell lysate. It is guaranteed to work for IP, IF, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2018-12-21

Question

Is a blocking peptide available for product anti-Vinculin Rabbit Monoclonal antibody (M01207)?

Verified Customer

Verified customer

Asked: 2018-08-10

Answer

We do provide the blocking peptide for product anti-Vinculin Rabbit Monoclonal antibody (M01207). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-08-10

Question

Please see the WB image, lot number and protocol we used for uterus using anti-Vinculin Rabbit Monoclonal antibody M01207. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-04-05

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-04-05

Question

We are interested in to test anti-Vinculin Rabbit Monoclonal antibody M01207 on human uterus for research purposes, then I may be interested in using anti-Vinculin Rabbit Monoclonal antibody M01207 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

E. Jha

Verified customer

Asked: 2018-02-05

Answer

The products we sell, including anti-Vinculin Rabbit Monoclonal antibody M01207, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-02-05

Question

Does anti-Vinculin Rabbit Monoclonal antibody M01207 work for WB with uterus?

Verified Customer

Verified customer

Asked: 2017-10-23

Answer

According to the expression profile of uterus, VCL is highly expressed in uterus. So, it is likely that anti-Vinculin Rabbit Monoclonal antibody M01207 will work for WB with uterus.

Boster Scientific Support

Answered: 2017-10-23

Question

We are currently using anti-Vinculin Rabbit Monoclonal antibody M01207 for rat tissue, and we are content with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2017-09-29

Answer

The anti-Vinculin Rabbit Monoclonal antibody (M01207) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-09-29

Question

I see that the anti-Vinculin Rabbit Monoclonal antibody M01207 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2017-08-22

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-08-22

Question

Can you help my question with product M01207, anti-Vinculin Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

A. Carter

Verified customer

Asked: 2015-11-17

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01207 anti-Vinculin Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2015-11-17

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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In stock
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M01207
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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