Click image to see more details
Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
|Application:||IP, IF, WB|
Customers Who Bought This Also Bought
Anti-Vinculin VCL Rabbit Monoclonal Antibody
Boster Bio Anti-Vinculin VCL Rabbit Monoclonal Antibody catalog # M01207. Tested in IP, IF, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Vinculin VCL Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01207)
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
A synthesized peptide derived from human Vinculin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
M01207 is reactive to VCL in Human, Mouse, Rat
M01207 is guaranteed for IP, IF, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.
Submit A Review
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Vinculin expression in (1) HepG2 cell lysate; (2) HeLa cell lysate (M01207).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VCL monoclonal antibody (Catalog # M01207) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VCL
Gene/Protein Information For VCL (Source: Uniprot.Org, NCBI)
CMD1W; CMH15; metavinculin; MVCL; VCL; Vinculin VCL CMD1W, CMH15, HEL114, MV, MVCL vinculin vinculin|epididymis luminal protein 114|epididymis secretory sperm binding protein|meta-vinculin|metavinculin|vinculin isoform meta-VCL*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on VCL, check out the VCL Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for VCL: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
M01207 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Wang K,Xing ZH,Jiang QW,Yang Y,Huang JR,Yuan ML,Wei MN,Li Y,Wang ST,Liu K,Shi Z.Targeting uPAR by CRISPR/Cas9 System Attenuates Cancer Malignancy and Multidrug Resistance.Front Oncol.2019 Feb 27;9:80.doi:10.3389/fonc.2019.00080.PMID:30873379;PMCID:PMC6400983.
M01207 usage in article: APP:WB, SAMPLE:HCT8/T CELL AND KBV200 CELL, DILUTION:NA
Yao Q, An Y, Hou W, Cao YN, Yao MF, Ma NN, Hou L, Zhang H, Liu HJ, Zhang B. Oncotarget. 2017 Nov 30;8(65):109632-109645. doi: 10.18632/oncotarget.22759. eCollection 2017 Dec 12. LRP6 promotes invasion and metastasis of colorectal cancer through cy...
Shen Z, Lu D, Li Q, Zhang Z, Zhu Y. Biomed Res Int. 2015;2015:871202. Doi: 10.1155/2015/871202. Epub 2015 Mar 8. Synthesis And Characterization Of Biodegradable Polyurethane For Hypopharyngeal Tissue Engineering.
Su F,, Liu Ss,, Ma Jl, Wang Ds, E Ll, Liu Hc. Stem Cell Res Ther. 2015 Mar 12;6:22. Doi: 10.1186/S13287-015-0023-3. Enhancement Of Periodontal Tissue Regeneration By Transplantation Of Osteoprotegerin-Engineered Periodontal Ligament Stem Cells.
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Have you used Anti-Vinculin VCL Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Submit A Review
Be the first to review Anti-Vinculin VCL Rabbit Monoclonal Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question