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Product Info Summary
| SKU: | A11391 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-VLK Pkdcc Antibody
View all Protein kinase-like protein SgK493 Antibodies
SKU/Catalog Number
A11391
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-VLK Pkdcc Antibody (Catalog # A11391). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
VLK antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-VLK Pkdcc Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11391)
Host
Rabbit
Contents
VLK Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
VLK antibody was raised against a 15 amino acid synthetic peptide from near the center of human VLK. The immunogen is located within amino acids 320 - 370 of VLK.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
AMIGO1 antibody is human, mouse, and rat reactive. AMIGO1 antibody is predicted to not cross-react with AMIGO2 or AMIGO3.
Reactive Species
A11391 is reactive to PKDCC in Human, Mouse, Rat
Applications
A11391 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
54.132kDa
Background of Protein kinase-like protein SgK493
VLK was identified as a novel protein kinase that was induced after the differentiation of cultured embryonic stem cells into mesendoderm. It has no homologs in invertebrates, but is highly conserved in vertebrate species although it does not belong to any known protein kinase groups. VLK is initially expressed in E-cadherin-positive anterior visceral endoderm and mesendoderm, but its expression is later confined to E-cadherin-negative mesenchyme. It is enriched in the Golgi apparatus and is thought to regulate the rate of protein export from the Golgi. Targeted disruption of VLK in mice leads to a defect in lung development and neonatal lethality. It has been suggested that mutations in VLK may be associated with the allergic condition atopy.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
VLK antibody can be used for detection of VLK by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of VLK in human lung tissue lysate with VLK antibody at 1 μg/mL.
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Immunohistochemistry of VLK in rat lung tissue with VLK antibody at 2.5 μg/mL.
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Immunofluorescence of VLK in rat lung tissue with VLK antibody at 5 μg/mL.
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Western blot analysis of VLK expression in Jurkat cell lysate with VLK antibody at 0.5 μg/ml.
Protein Target Info & Infographic
Gene/Protein Information For PKDCC (Source: Uniprot.org, NCBI)
Gene Name
PKDCC
Full Name
Extracellular tyrosine-protein kinase PKDCC
Weight
54.132kDa
Superfamily
protein kinase superfamily
Alternative Names
EC 2.7; FLJ18197; MGC125960; protein kinase domain containing, cytoplasmic homolog (mouse); protein kinase domain-containing protein, cytoplasmic; Protein kinase-like protein SgK493; SgK493; Sugen kinase 493; Vertebrate lonesome kinase; Vlk PKDCC RLSDF, SGK493, Vlk protein kinase domain containing, cytoplasmic extracellular tyrosine-protein kinase PKDCC|protein kinase domain containing, cytoplasmic homolog|protein kinase domain-containing protein, cytoplasmic|protein kinase-like protein SgK493|sugen kinase 493|vertebrate lonesome kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PKDCC, check out the PKDCC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PKDCC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-VLK Pkdcc Antibody (A11391)
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