Anti-VNN1 Antibody Picoband™

Product Name

Anti-VNN1 Antibody Picoband™

View all Vanin-1/VNN1 Antibodies

SKU/Catalog Number

PB10106

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-VNN1 Antibody Picoband™ catalog # PB10106. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-VNN1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10106)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human VNN1 recombinant protein (Position: Q22-K192). Human VNN1 shares 82.2% amino acid (aa) sequence identity with mouse VNN1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

PB10106 is reactive to VNN1 in Human

Applications

PB10106 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

57 kDa

Calculated molecular weight

57.012kDa

Background of Vanin-1/VNN1

Pantetheinase is an enzyme that in humans is encoded by the VNN1 gene. This gene encodes a member of the vanin family of proteins, which share extensive sequence similarity with each other, and also with biotinidase. The family includes secreted and membrane-associated proteins, a few of which have been reported to participate in hematopoietic cell trafficking. No biotinidase activity has been demonstrated for any of the vanin proteins, however, they possess pantetheinase activity, which may play a role in oxidative-stress response. This protein, like its mouse homolog, is likely a GPI-anchored cell surface molecule. The mouse protein is expressed by the perivascular thymic stromal cells and regulates migration of T-cell progenitors to the thymus. This gene lies in close proximity to, and in the same transcriptional orientation as, two other vanin genes on chromosome 6q23-q24.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of VNN1 using anti-VNN1 antibody (PB10106).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: SKOV3 whole cell lysates,
Lane 2: HELA whole cell lysates lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VNN1 antigen affinity purified polyclonal antibody (Catalog # PB10106) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VNN1 at approximately 57 kDa. The expected band size for VNN1 is at 57 kDa.

Gene/Protein Information For VNN1 (Source: Uniprot.org, NCBI)

Alternative Names

EC 3.5.1; EC 3.5.1.92; HDLCQ8; MGC116930; MGC116931; MGC116932; Pantetheinase; Pantetheine hydrolase; Tiff66; Tiff66MGC116933; vanin 1; Vanin1; Vanin-1; vannin 1; Vascular non-inflammatory molecule 1; VNN1 VNN1 HDLCQ8, Tiff66 vanin 1 pantetheinase|pantetheine hydrolase|vannin 1|vascular non-inflammatory molecule 1

For more info on VNN1, check out the VNN1 Infographic

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In this infographic, you will see the following information for VNN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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