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Product Info Summary
| SKU: | M00270-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-VWF/Von Willebrand Factor Rabbit Monoclonal Antibody
SKU/Catalog Number
M00270-1
BM4860 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-VWF/Von Willebrand Factor Rabbit Monoclonal Antibody catalog # M00270-1. Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-VWF/Von Willebrand Factor Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00270-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
IHF-22
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human VWF
Reactive Species
M00270-1 is reactive to VWF in Human
Observed Molecular Weight
309 kDa
Calculated molecular weight
309.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00270-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
Positive Control
WB: human serum
Validation Images & Assay Conditions
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Western blot analysis of VWF expression in human serum lysate.
Specific Publications For Anti-VWF/Von Willebrand Factor Rabbit Monoclonal Antibody (M00270-1)
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Customer Q&As
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3 Customer Q&As for Anti-VWF/Von Willebrand Factor Rabbit Monoclonal Antibody
Question
My team were satisfied with the WB result of your anti-VWF/Von Willebrand Factor Rabbit Monoclonal antibody. However we have been able to see positive staining in liver secreted. using this antibody. Is that expected? Could you tell me where is VWF supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
According to literature, liver does express VWF. Generally VWF expresses in secreted. Regarding which tissues have VWF expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Lung, Pubmed ID: 15489334
Plasma, Pubmed ID: 14760718
Platelet, Pubmed ID: 12665801
Umbilical vein endothelial cell, Pubmed ID: 3495266
Boster Scientific Support
Answered: 2020-02-10
Question
We are currently using anti-VWF/Von Willebrand Factor Rabbit Monoclonal antibody M00270-1 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2018-11-23
Answer
The anti-VWF/Von Willebrand Factor Rabbit Monoclonal antibody (M00270-1) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-11-23
Question
We have observed staining in human liver. Are there any suggestions? Is anti-VWF/Von Willebrand Factor Rabbit Monoclonal antibody supposed to stain liver positively?
D. Miller
Verified customer
Asked: 2016-04-27
Answer
From literature liver does express VWF. From Uniprot.org, VWF is expressed in lung, umbilical vein endothelial cell, platelet, plasma, liver, among other tissues. Regarding which tissues have VWF expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Lung, Pubmed ID: 15489334
Plasma, Pubmed ID: 14760718
Platelet, Pubmed ID: 12665801
Umbilical vein endothelial cell, Pubmed ID: 3495266
Boster Scientific Support
Answered: 2016-04-27
