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- Table of Contents
VWF · Western blot design guide
VWF Western Blot Planning Guide
Plan a VWF Western blot around the catalog-observed 309.3 kDa band, image-backed PB9062 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
VWF Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 309.3 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 4-12% gradient | |
| Positive control | Endometrium | |
| Negative control | Appendix |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 309.3 kDa |
| ⓘ | Localization | Secreted / Secreted, extracellular space, extracellular matrix |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Section 1
Real Curated VWF Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Endometrium |
| Gel % | 4-12% gradient |
| Load | 20-30 µg total protein per lane |
| Transfer | Wet/tank, extended transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | PB9062 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected VWF Western Blot Band Size?
Use the product-observed 309.3 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 309.3 kDa | Matches the authoritative product WB observation. |
| 309.3 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected VWF appearancePlan around 309.3 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 309.3 kDa; use this as the primary experimental expectation. |
| Calculated mass | 309.3 kDa from UniProt P04275; retain as context. |
| Gel selection | 4-12% gradient; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with PB9062. |
| Situation | Likely cause | Next action |
|---|---|---|
| 309.3 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing VWF band
PB9062 · VWF
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for VWF Western blot
🧪Use Endometrium as the first positive-control candidate and Appendix as the HPA Not detected negative candidate.
Positive control: Endometrium (High)
Negative control: Appendix (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for VWF
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Section 3
Advanced VWF Western Blot Tips
Deeper troubleshooting and optimisation questions for VWF, answered from its protein features.
Which band should guide the blot?
Use 309.3 kDa, the observation attached to the authoritative PB9062 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 309.3 kDa expectation.
Which positive control should I start with?
Start with Endometrium, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Appendix as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 4-12% gradient consistently across the quick facts, protocol table, and poster.
What transfer method to use for VWF Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should PB9062 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
VWF Western Blot Reagents
Human/Mouse/Rat-reactive VWF Western blot reagents with authoritative product imagery.
Real WB data
Anti-Von Willebrand Factor/VWF Antibody Picoband®
Cat # PB9062
Real WB data
Anti-Von Willebrand Factor/VWF Antibody Picoband®
Cat # PB9273
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt P04275
- Human Protein Atlas — VWF
- PB9062 product record
- PMC12500436 — Macrophage Tim-4 protects against deep vein thrombosis by binding CK2β to suppress inflammatory responses (Frontiers in immunology, 2025)
- PMC3323834 — Modulation of the von Willebrand factor-dependent platelet adhesion through alternative proteolytic pathways (Thrombosis research, 2012)
- PMC423489 — Subunit composition of plasma von Willebrand factor. Cleavage is present in normal individuals, increased in IIA and IIB von Willebrand disease, but minimal in variants with aberrant structure of individual oligomers (types IIC, IID, and IIE) (The Journal of clinical investigation, 1986)
