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Product Info Summary
| SKU: | A06240 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-WDHD1 Antibody
SKU/Catalog Number
A06240
Size
100μl
Form
Liquid
Description
Boster Bio Anti-WDHD1 Antibody catalog # A06240. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-WDHD1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06240)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human WDHD1. AA range:721-770
Reactive Species
A06240 is reactive to WDHD1 in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
126.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06240 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Validation Images & Assay Conditions
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Western blot analysis of lysates from Jurkat and HepG2 cells, using WDHD1 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4° overnight.3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Specific Publications For Anti-WDHD1 Antibody (A06240)
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-WDHD1 Antibody
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-WDHD1 antibody A06240. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-17
Question
We are currently using anti-WDHD1 antibody A06240 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-29
Answer
The anti-WDHD1 antibody (A06240) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-29
Question
I see that the anti-WDHD1 antibody A06240 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-03-26
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-03-26
Question
Would anti-WDHD1 antibody A06240 work on bovine WB with fetal brain?
R. Roberts
Verified customer
Asked: 2015-01-05
Answer
Our lab technicians have not tested anti-WDHD1 antibody A06240 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-WDHD1 antibody A06240 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine fetal brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-01-05
