Anti-WDR36 Antibody Picoband™

Product Name

Anti-WDR36 Antibody Picoband™

View all WDR36 Antibodies

SKU/Catalog Number

A05779-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-WDR36 Antibody Picoband™ catalog # A05779-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-WDR36 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05779-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human WDR36 recombinant protein (Position: R74-D865).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

A05779-1 is reactive to WDR36 in Human

Applications

A05779-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

105 kDa

Calculated molecular weight

105.322kDa

Background of WDR36

WD repeat-containing protein 36 is a protein that in humans is encoded by the WDR36 gene. This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Mutations in this gene have been associated with adult-onset primary open-angle glaucoma (POAG).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Flow Cytometry, 1-3 µg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of WDR36 using anti-WDR36 antibody (A05779-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Daudi whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WDR36 antigen affinity purified polyclonal antibody (Catalog # A05779-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WDR36 at approximately 105 kDa. The expected band size for WDR36 is at 105 kDa.

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Figure 2. IF analysis of WDR36 using anti-WDR36 antibody (A05779-1) and anti-Tubulin Alpha antibody (M03989-3).
WDR36 was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-WDR36 Antibody (A05779-1) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight594 Conjugated Goat Anti-Rabbit IgG (BA1142) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Figure 3. Flow Cytometry analysis of A431 cells using anti-WDR36 antibody (A05779-1).
Overlay histogram showing A431 cells stained with A05779-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-WDR36 Antibody (A05779-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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Figure 4. Flow Cytometry analysis of U251 cells using anti-WDR36 antibody (A05779-1).
Overlay histogram showing U251 cells stained with A05779-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-WDR36 Antibody (A05779-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For WDR36 (Source: Uniprot.org, NCBI)

Alternative Names

DKFZp686I1650; TA-WDRPopen angle, G; WD repeat domain 36; WD repeat-containing protein 36 WDR36 GLC1G, TA-WDRP, TAWDRP, UTP21 WD repeat domain 36 WD repeat-containing protein 36|T-cell activation WD repeat protein|T-cell activation WD repeat-containing protein

For more info on WDR36, check out the WDR36 Infographic

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In this infographic, you will see the following information for WDR36: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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