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Product Info Summary
| SKU: | A12524 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-WDR61 Antibody
SKU/Catalog Number
A12524
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-WDR61 Antibody (Catalog # A12524). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
WDR61 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-WDR61 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12524)
Host
Rabbit
Contents
WDR61 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
WDR61 antibody was raised against a 14 amino acid peptide near the amino terminus of human WDR61. The immunogen is located within the first 50 amino acids of WDR61.
Cross-reactivity
WDR61 antibody is human, mouse and rat reactive.
Reactive Species
A12524 is reactive to WDR61 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
33.6 kDa
Background of WDR61
WDR61 (WD-repeat-containing protein 61), also known as SKI8 or REC14, is a subunit of the human PAF and SKI complexes, which function in transcriptional regulation and are involved in events downstream of RNA synthesis, such as RNA surveillance (1,2). PAF1C is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1C (3,4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12524 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WDR61 antibody can be used for detection of WDR61 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of WDR61 in SK-N-SH cell lysate with WDR61 antibody at 1 μg/ml.
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Immunohistochemistry of WDR61 in mouse brain tissue with WDR61 antibody at 5 μg/ml.
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Immunofluorescence of WDR61 in mouse brain tissue with WDR61 antibody at 20 μg/ml.
Specific Publications For Anti-WDR61 Antibody (A12524)
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