Anti-Wnt-1 Monoclonal Antibody

Product Name

Anti-Wnt-1 Monoclonal Antibody

View all Wnt-1 Antibodies

SKU/Catalog Number

M00354

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Wnt-1 Monoclonal Antibody catalog # M00354. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Wnt-1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00354)

Host

Mouse

Contents

Ascitic fluid containing 0.03% sodium azide.

Clonality

Monoclonal

Clone Number

10C8

Isotype

IgG

Immunogen

Purified recombinant fragment of Wnt-1 expressed in E. Coli.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00354 is reactive to WNT1 in Human, Mouse

Applications

M00354 is guaranteed for ELISA, Flow Cytometry, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

40.982kDa

Background of Wnt-1

Paired box protein Pax-6 also known as aniridia type II protein (AN2) or oculorhombin is a transcription factor with key functions in the development of the eye, nose, central nervous system and pancreas. Pax6 further seems to be required for the differentiation of pancreatic islet alpha cells. PAX6 has been shown to bind to a promoter element in the glucagon, insulin and somatostatin promoters and regulates the specification of the ventral neuron subtypes by establishing the correct progenitor domains. Isoform 5a appears to function as a molecular switch that specifies target genes. Anti-PAX6 antibody is ideal for researchers interested in developmental biology, stem cell research, cell growth and cancer research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:200-1:1000
IF 1:200-1:1000
FC 1:200-1:400
ELISA 1:10000

Validation Images & Assay Conditions

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Figure 3. Flow Cytometry validation of WNT1 using Anti-Wnt-1 Monoclonal Antibody (M00354).

Flow cytometric (FCM) analysis of HeLa cells using Wnt-1 Monoclonal Antibody (green) and negative control (purple).

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Figure 4. Immunofluorescent staining data of WNT1 using Anti-Wnt-1 Monoclonal Antibody (M00354).

Confocal Immunofluorescence (IF) analysis of HeLa (left) and 3T3-L1 (right) cells using Wnt-1 Monoclonal Antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

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Figure 2. Immunohistochemistry validation of WNT1 using Anti-Wnt-1 Monoclonal Antibody (M00354).

Immunohistochemistry (IHC) analysis of paraffin-embedded human Adrenal tissues with AEC staining using Wnt-1 Monoclonal Antibody.
For more protocol information of IHC

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Figure 1. Western blotting validation for Anti-Wnt-1 Monoclonal Antibody M00354

Western Blot (WB) analysis using Wnt-1 Monoclonal Antibody against NIH/3T3 (1)

Gene/Protein Information For WNT1 (Source: Uniprot.org, NCBI)

Alternative Names

Int-1; INT1Proto-oncogene Int-1 homolog; proto-oncogene Wnt-1; wingless-type MMTV integration site family, member 1 (oncogene INT1); wingless-type MMTV integration site family, member 1; Wnt1; Wnt-1 WNT1 BMND16, INT1, OI15 Wnt family member 1 proto-oncogene Wnt-1|proto-oncogene Int-1 homolog|wingless-type MMTV integration site family member 1|wingless-type MMTV integration site family, member 1 (oncogene INT1)

For more info on WNT1, check out the WNT1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for WNT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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