Anti-Werner's syndrome helicase WRN/WRN Antibody Picoband™

Product Name

Anti-Werner's syndrome helicase WRN/WRN Antibody Picoband™

View all WRN Antibodies

SKU/Catalog Number

PB10107

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Werner's syndrome helicase WRN/WRN Antibody Picoband™ catalog # PB10107. Tested in WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Werner's syndrome helicase WRN/WRN Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10107)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human WRN recombinant protein (Position: Q122-N240). Human WRN shares 84% amino acid (aa) sequence identity with mouse WRN.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

PB10107 is reactive to WRN in Human, Rat

Applications

PB10107 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

162 kDa, 200 kDa

Calculated molecular weight

162.461kDa

Background of WRN

Werner syndrome ATP-dependent helicase, also known as DNA helicase, RecQ-like type 3, is an enzyme that in humans is encoded by the WRN gene. This gene encodes a member of the RecQ subfamily and the DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNA helicases are involved in many aspects of DNA metabolism, including transcription, replication, recombination, and repair. This protein contains a nuclear localization signal in the C-terminus and shows a predominant nucleolar localization. It possesses an intrinsic 3' to 5' DNA helicase activity, and is also a 3' to 5' exonuclease. Based on interactions between this protein and Ku70/80 heterodimer in DNA end processing, this protein may be involved in the repair of double strand DNA breaks. Defects in this gene are the cause of Werner syndrome, an autosomal recessive disorder characterized by premature aging.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of WRN using anti-WRN antibody (PB10107).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat thymus tissue lysates,
Lane 2: human placenta tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WRN antigen affinity purified polyclonal antibody (Catalog # PB10107) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WRN at approximately 162kDa, 200 kDa. The expected band size for WRN is at 162 kDa.

Gene/Protein Information For WRN (Source: Uniprot.org, NCBI)

Alternative Names

DKFZp686C2056; DNA helicase, RecQ-like type 3; EC 3.1; EC 3.6.1; EC 3.6.4.12; Exonuclease WRN; RecQ protein-like 2; RecQ3; RECQ3RECQL3; RECQL2Werner syndrome ATP-dependent helicase; Werner syndrome; Werner syndrome, RecQ helicase-like WRN RECQ3, RECQL2, RECQL3 WRN RecQ like helicase Werner syndrome ATP-dependent helicase|DNA helicase, RecQ-like type 3|Werner syndrome RecQ like helicase|Werner syndrome, RecQ helicase-like|exonuclease WRN|recQ protein-like 2

For more info on WRN, check out the WRN Infographic

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In this infographic, you will see the following information for WRN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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