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Product Info Summary
| SKU: | A03432 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-XAF-1 Antibody
SKU/Catalog Number
A03432
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-XAF-1 Antibody (Catalog # A03432). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
XAF-1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-XAF-1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03432)
Host
Rabbit
Contents
XAF-1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
XAF-1 antibody was raised against a synthetic peptide corresponding to amino acids at the C-terminus of human XAF-1. The immunogen is located within the last 50 amino acids of XAF-1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
KLK4 antibody is human specific. At least three isoforms of KLK4 are known to exist.
Reactive Species
A03432 is reactive to XAF1 in Human, Mouse
Applications
A03432 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
34.626kDa
Background of XAF1
XAF-1 binds to XIAP, an inhibitor of caspases-3, -7, and -9, and triggers its relocation from the cytosol to the nucleus. Overexpression of XAF-1 results in the neutralization of XIAP's ability to inhibit cell death. XAF-1 is normally expressed in all adult and fetal tissues but was found to be present in very low levels in a variety of cancer cell lines. In contrast, XIAP levels have been shown to be high in a majority of cell lines. Low XAF-1 and high basal expression of XIAP may therefore play a critical role in maintaining survival of cancer cell lines. Both IFN-alpha2 and IFN-beta can induce XAF-1 mRNA in all cells examined but induction of XAF-1 protein (as observed by immunoblot analysis) was seen only in cell lines sensitive to the apoptotic effects of IFNs.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
XAF-1 antibody can be used for the detection of XAF-1 by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of XAF-1 in human spleen lysate with XAF-1 antibody at (A) 0.5
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Immunohistochemical staining of human spleen tissue using XAF-1 antibody at 2 μg/mL.
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Immunofluorescence of XAF-1 in Human Spleen tissue with XAF-1 antibody at 10 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For XAF1 (Source: Uniprot.org, NCBI)
Gene Name
XAF1
Full Name
XIAP-associated factor 1
Weight
34.626kDa
Alternative Names
BIRC4-binding protein; BIRC4BPBIRC4 binding protein; HSXIAPAF1; XIAP associated factor 1; XIAPAF1XIAP-associated factor 1 XAF1 BIRC4BP, HSXIAPAF1, XIAPAF1 XIAP associated factor 1 XIAP-associated factor 1|BIRC4-binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on XAF1, check out the XAF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for XAF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-XAF-1 Antibody (A03432)
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No publications found for A03432
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Customer Reviews
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