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Product Info Summary
| SKU: | M03036 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, ICC, WB |
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Product info
Product Name
Anti-xCT Rabbit Monoclonal Antibody
SKU/Catalog Number
M03036
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-xCT Rabbit Monoclonal Antibody catalog # M03036. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-xCT Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03036)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ACGI-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human xCT
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03036 is reactive to SLC7A11 in Human, Mouse, Rat
Applications
M03036 is guaranteed for IP, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
56 kDa
Calculated molecular weight
55.423kDa
Background of xCT
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:150
IP 1:50
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1.5W dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1.5W dilution for 1 hour at room temperature.
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Western blot analysis of xCT expression in (1) HepG2 cell lysate; (2) Mouse brain lysate.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
Protein Target Info & Infographic
Gene/Protein Information For SLC7A11 (Source: Uniprot.org, NCBI)
Gene Name
SLC7A11
Full Name
Cystine/glutamate transporter
Weight
55.423kDa
Superfamily
amino acid-polyamine-organocation (APC) superfamily
Alternative Names
Amino acid transport system xc-; Calcium channel blocker resistance protein CCBR1; CCBR1; cystine/glutamate transporter; Member 11 (Slc7a11); SLC7A11; Solute carrier family 7 member 11; solute carrier family 7, (cationic amino acid transporter, y+ system) member 11; xCT; xCTCCBR1 SLC7A11 CCBR1, xCT solute carrier family 7 member 11 cystine/glutamate transporter|amino acid transport system xc-|calcium channel blocker resistance protein CCBR1|solute carrier family 7 (anionic amino acid transporter light chain, xc- system), member 11|solute carrier family 7, (cationic amino acid transporter, y+ system) member 11
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SLC7A11, check out the SLC7A11 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SLC7A11: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-xCT Rabbit Monoclonal Antibody (M03036)
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No publications found for M03036
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Customer Reviews
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Customer Q&As
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8 Customer Q&As for Anti-xCT Rabbit Monoclonal Antibody
Question
We are currently using anti-xCT Monoclonal antibody M03036 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
The anti-xCT Monoclonal antibody (M03036) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-31
Question
What sample target on Western Blot application was tested with M03036?
Verified customer
Asked: 2020-01-03
Answer
Western blot analysis of xCT expression in (1) HepG2 cell lysate; (2) Mouse brain lysate.
Boster Scientific Support
Answered: 2020-01-16
Question
Is there a BSA free version of anti-xCT Monoclonal antibody M03036 available?
Verified Customer
Verified customer
Asked: 2019-12-19
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-xCT Monoclonal antibody M03036 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-19
Question
Would M03036 anti-xCT Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
It shows on the product datasheet, M03036 anti-xCT Monoclonal antibody as been tested on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-08
Question
I was wanting to use your anti-xCT Monoclonal antibody for IP for human tongue on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human tongue identification?
Verified Customer
Verified customer
Asked: 2019-02-25
Answer
It shows on the product datasheet, M03036 anti-xCT Monoclonal antibody has been validated for IP, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human tongue in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-02-25
Question
I have attached the WB image, lot number and protocol we used for tongue using anti-xCT Monoclonal antibody M03036. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-12-01
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-12-01
Question
Is this M03036 anti-xCT Monoclonal antibody reactive to the isotypes of SLC7A11?
M. Gonzalez
Verified customer
Asked: 2017-05-12
Answer
The immunogen of M03036 anti-xCT Monoclonal antibody is A synthesized peptide derived from human xCT Sodium-independent, high-affinity exchange of anionic amino acids with high specificity for anionic form of cystine and glutamate. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-05-12
Question
Will anti-xCT Monoclonal antibody M03036 work on feline WB with placenta?
L. Zhao
Verified customer
Asked: 2016-04-01
Answer
Our lab technicians have not validated anti-xCT Monoclonal antibody M03036 on feline. You can run a BLAST between feline and the immunogen sequence of anti-xCT Monoclonal antibody M03036 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline placenta in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-04-01
