Mouse Gp130/IL6ST PicoKine™ Quick ELISA Kit

Mouse Gp130/IL6ST PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Il6st in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml.

Product Info Summary

SKU: FEK0577
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Assay Time: 90 Minutes

Introduction

Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.

Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol

Product Name

Mouse Gp130/IL6ST PicoKine™ Quick ELISA Kit

See all gp130/CD130 products

SKU/Catalog Number

FEK0577

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse Gp130/IL6ST PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Il6st in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse Gp130/IL6ST PicoKine™ Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0577)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q23-E617

Sensitivity

<10 pg/ml

Assay Range

125 pg/ml - 8,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0577 is reactive to Il6st in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Application Guarantee

FEK0577 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog number Description Quantity
FEK0577-CAP 96-well plate precoated with anti-Mouse gp130/CD130 antibody 1
FEK0577-ST lyophilized recombinant Mouse gp130/CD130 standard 10 ng/tube
FEK0577-DA Biotinylated anti-Mouse gp130/CD130 detection antibody (50x) 130 ul
- Avidin-Biotin-Peroxidase Complex (30x) 400 µl
AR1106-1 Sample diluent 30 ml
AR1106-2 Antibody diluent 12 ml
- Avidin-Biotin-Peroxidase Diluent 12 ml
AR1106-5 Wash Buffer (25x) 20 ml
AR1104 Color Developing Reagent (TMB) 10 ml
AR1105 Stop Solution 10 ml
PLA-SEA Plate Sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)08000200040008000200040008000
O.D.0.0880.1330.1990.3070.4840.8291.2541.911

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)1591404458815814734382
Standard deviation11.671.6339.5114.0681.01333.03
CV (%)7.3%7.6%%7.4%8.9%5.5%7.6%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of gp130/CD130 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 115918518516817411.216.4%
Sample 21404135212321273131566.975%
Sample 345884716442346744600112.252.4%
*number of samples for each test n=16.

Gene/Protein Information For Il6st (Source: Uniprot.Org, NCBI)

Uniprot ID

Q00560

Gene ID

16195

Gene Name

Il6st

Full Name

Interleukin-6 receptor subunit beta

Weight

102.451kDa

Superfamily

type I cytokine receptor family

Alternative Names

CD130 antigen; CD130; CDw130; DKFZp564F053; gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form; gp130; IL-6 receptor subunit beta; IL-6R subunit beta; IL-6RB; IL-6R-beta; IL6ST; interleukin 6 signal transducer (gp130, oncostatin M receptor); interleukin receptor beta chain; interleukin-6 receptor subunit beta; Interleukin-6 signal transducer; Membrane glycoprotein 130; membrane glycoprotein gp130; Oncostatin-M receptor subunit alpha Il6st|5133400A03Rik, AA389424, BB405851, CD130, D13Ertd699, D13Ertd699e, gp13, gp130|interleukin 6 signal transducer|interleukin-6 receptor subunit beta|IL-6 receptor subunit beta|IL-6R subunit beta|IL-6R-beta|IL-6RB|membrane glycoprotein 130|oncostatin-M receptor subunit alpha

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Il6st, check out the Il6st Infographic

Il6st infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Il6st: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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