Anti-ZBTB4 Antibody

Product Name

Anti-ZBTB4 Antibody

View all ZBTB4 Antibodies

SKU/Catalog Number

A08155

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-ZBTB4 Antibody (Catalog # A08155). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-ZBTB4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08155)

Host

Rabbit

Contents

ZBTB4 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

ZBTB4 antibody was raised against a 18 amino acid synthetic peptide near the carboxy terminus of human ZBTB4. The immunogen is located within amino acids 790 - 840 of ZBTB4.

Cross-reactivity

At least four isoforms of ZBTB4 are known to exist; this antibody will only recognize the longest isoform. This antibody is predicted to not cross-react with other ZBTB protein family members.

Reactive Species

A08155 is reactive to ZBTB4 in Human, Mouse

Observed Molecular Weight

68 kDa

Calculated molecular weight

105.1 kDa

Background of ZBTB4

The ZBTB family of proteins is comprised of diverse zinc finger proteins that also contain a BTB (BR-C, ttk and bab) domain. Similar to Kaiso, a zinc-finger containing protein that can bind methylated CpGs, ZBTB4 can also bind methylated DNA and repress transcription. ZBTB4 has been shown to associate with the Sin3/histone deacetylase co-repressor and repress expression of P21CIP1 as part of a heterodimeric complex with Miz1. In cultured cells, depletion of ZBTB4 promotes cell cycle arrest in response to p53 activation and suppresses apoptosis through regulation of P21CIP1, suggesting that ZBTB4 is a critical determinant of the cellular response to p53 activation. HIPK2, a kinase that is involved in cellular proliferation and survival, phosphorylates and down-regulates ZBTB4 under normal cell growth conditions; this degradation increases with DNA damage.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A08155 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ZBTB4 antibody can be used for detection of ZBTB4 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information ZBTB4

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