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- Table of Contents
TUBA1A antibody (AZQ6NWK7) performs well in IF, IHC, WB and shows reactivity with Zebrafish.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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IF analysis of Alpha Tubulin/TUBA1A using anti-Alpha Tubulin/TUBA1A antibody (AZQ6NWK7).
Alpha Tubulin/TUBA1A was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Alpha Tubulin/TUBA1A Antibody (AZQ6NWK7) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Alpha Tubulin/TUBA1A was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Alpha Tubulin/TUBA1A Antibody (AZQ6NWK7) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-Zebrafish Alpha Tubulin/TUBA1A Antibody Picoband®
TUBA1A antibody
| Reactive Species | Host | Application |
|---|---|---|
| Zebrafish | Rabbit | IF, IHC, WB |
Tested Applications
TUBA1A antibody was positively detected by WB in:
zebrafish head tissue, zebrafish embryo tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
TUBA1A antibody was positively detected by IHC in:
zebrafish brain tissue, zebrafish spinal cord tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
TUBA1A antibody was positively detected by IF in:
zebrafish embryo tissue
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Zebrafish
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Zebrafish
Immunofluorescence
2 μg/ml
Zebrafish
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Zebrafish Alpha Tubulin/TUBA1A Antibody Picoband®
View all TUBA1A Antibodies
View all TUBA1A Antibodies
Form
Lyophilized
Uniprot
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish Alpha Tubulin/TUBA1A recombinant protein (Position: M1-G436).
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Background of TUBA1A
Predicted to enable GTP binding activity. Predicted to be a structural constituent of cytoskeleton. Acts upstream of or within negative regulation of apoptotic process and optic nerve formation. Predicted to be located in cytoskeleton. Predicted to be active in cytoplasm and microtubule. Human ortholog(s) of this gene implicated in lissencephaly; lissencephaly 3; and microcephaly. Orthologous to several human genes including TUBA1A (tubulin alpha 1a).
Cite This Product
Anti-Zebrafish Alpha Tubulin/TUBA1A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZQ6NWK7)
Protocols
Western Blot protocol for TUBA1A antibody
Immunohistochemistry (IHC) protocol for TUBA1A antibody
Immunofluorescence (IF) protocol for TUBA1A antibody
Publications
Application:
Species:
From Molecular Perturbations to Functional Decline: Multi-Omics Reveals Sperm Cryodamage in Sichuan Bream (Sinibrama taeniatus)
Published: 2026-03-26
Journal: Animals
Application: WB
Impact Factor: 2.70
Species: Sichuan bream
PMID: 41975993
Review
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Product Promise
We promise Anti-Zebrafish Alpha Tubulin/TUBA1A Antibody Picoband® performs excellently in relevant applications for IF, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
AZQ6NWK7
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$470.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 14, 2026