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Product Info Summary
| SKU: | AZQ7T2L8 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Zebrafish DUSP6 Antibody Picoband®
SKU/Catalog Number
AZQ7T2L8
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zebrafish DUSP6 Antibody Picoband® catalog # AZQ7T2L8. Tested in WB, IHC, IF applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Zebrafish DUSP6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZQ7T2L8)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish DUSP6 recombinant protein (Position: M1-K233).
Reactive Species
AZQ7T2L8 is reactive to DUSP6 in Zebrafish
Background of DUSP6
Enables phosphoprotein phosphatase activity. Acts upstream of or within several processes, including negative regulation of cardiac muscle tissue regeneration; regulation of endodermal cell fate specification; and regulation of signal transduction. Predicted to be active in cytosol. Is expressed in several structures, including brain; fin; germ ring; head; and pleuroperitoneal region. Human ortholog(s) of this gene implicated in hypogonadotropic hypogonadism 19 with or without anosmia. Orthologous to human DUSP6 (dual specificity phosphatase 6).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
AZQ7T2L8 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Zebrafish
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Zebrafish
Immunofluorescence, 2 μg/ml, Zebrafish
Validation Images & Assay Conditions
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Western blot analysis of DUSP6 using anti-DUSP6 antibody (AZQ7T2L8).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebrafish head tissue lysates,
Lane 2: zebrafish embryo tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DUSP6 antigen affinity purified polyclonal antibody (AZQ7T2L8) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DUSP6 at approximately 42 kDa. The expected band size for DUSP6 is at 42 kDa.
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IHC analysis of DUSP6 using anti-DUSP6 antibody (AZQ7T2L8).
DUSP6 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DUSP6 Antibody (AZQ7T2L8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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IF analysis of DUSP6 using anti-DUSP6 antibody (AZQ7T2L8).
DUSP6 was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-DUSP6 Antibody (AZQ7T2L8) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Specific Publications For Anti-Zebrafish DUSP6 Antibody Picoband® (AZQ7T2L8)
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