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DUSP6 antibody (AZQ7T2L8) performs well in IF, IHC, WB and shows reactivity with Zebrafish.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IF analysis of DUSP6 using anti-DUSP6 antibody (AZQ7T2L8).
DUSP6 was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-DUSP6 Antibody (AZQ7T2L8) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
DUSP6 was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-DUSP6 Antibody (AZQ7T2L8) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Designing a Western blot or IHC for DUSP6? The guides cover the expected band size and staining pattern, antibodies backed by real images, the controls to run, and protocols from published papers.
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Anti-Zebrafish DUSP6 Antibody Picoband®
DUSP6 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Zebrafish | Rabbit | IF, IHC, WB |
Tested Applications
DUSP6 antibody was positively detected by WB in:
zebrafish head tissue, zebrafish embryo tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
DUSP6 antibody was positively detected by IHC in:
zebrafish brain tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
DUSP6 antibody was positively detected by IF in:
zebrafish embryo tissue
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Zebrafish
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Zebrafish
Immunofluorescence
2 μg/ml
Zebrafish
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Zebrafish DUSP6 Antibody Picoband®
View all DUSP6 Antibodies
View all DUSP6 Antibodies
Form
Lyophilized
Uniprot
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish DUSP6 recombinant protein (Position: M1-K233).
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Background of DUSP6
Enables phosphoprotein phosphatase activity. Acts upstream of or within several processes, including negative regulation of cardiac muscle tissue regeneration; regulation of endodermal cell fate specification; and regulation of signal transduction. Predicted to be active in cytosol. Is expressed in several structures, including brain; fin; germ ring; head; and pleuroperitoneal region. Human ortholog(s) of this gene implicated in hypogonadotropic hypogonadism 19 with or without anosmia. Orthologous to human DUSP6 (dual specificity phosphatase 6).
Cite This Product
Anti-Zebrafish DUSP6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZQ7T2L8)
Protocols
Western Blot protocol for DUSP6 antibody
Immunohistochemistry (IHC) protocol for DUSP6 antibody
Immunofluorescence (IF) protocol for DUSP6 antibody
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Product Promise
We promise Anti-Zebrafish DUSP6 Antibody Picoband® performs excellently in relevant applications for IF, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
AZQ7T2L8
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$470.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 26, 2026