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Product Info Summary
| SKU: | AZQ6NW63 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-Zebrafish GATA6 Antibody
SKU/Catalog Number
AZQ6NW63
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zebrafish GATA6 Antibody catalog # AZQ6NW63. Tested in IHC applications. This antibody reacts with Zebrafish.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Zebrafish GATA6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZQ6NW63)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish GATA6 recombinant protein (Position: E26-E296)
Reactive Species
AZQ6NW63 is reactive to GATA6 in Zebrafish
Background of GATA6
Enables DNA binding activity. Acts upstream of or within several processes, including cardioblast migration to the midline involved in heart field formation; liver development; and positive regulation of transcription by RNA polymerase II. Predicted to be active in nucleus. Is expressed in several structures, including blastoderm; cardiovascular system; digestive system; mesoderm; and yolk syncytial layer. Human ortholog(s) of this gene implicated in adenocarcinoma (multiple); congenital heart disease (multiple); and pancreatic hypoplasia-diabetes-congenital heart disease syndrome. Orthologous to human GATA6 (GATA binding protein 6).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
AZQ6NW63 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Zebrafish
Validation Images & Assay Conditions
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IHC analysis of GATA6 using anti-GATA6 antibody (AZQ6NW63).
GATA6 was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GATA6 Antibody (AZQ6NW63) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Zebrafish GATA6 Antibody (AZQ6NW63)
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