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Product Info Summary
| SKU: | AZQ5MD90 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-Zebrafish GDF9 Antibody
SKU/Catalog Number
AZQ5MD90
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zebrafish GDF9 Antibody catalog # AZQ5MD90. Tested in IHC applications. This antibody reacts with Zebrafish.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Zebrafish GDF9 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZQ5MD90)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish GDF9 recombinant protein (Position: T295-R418).
Reactive Species
AZQ5MD90 is reactive to GDF9 in Zebrafish
Background of GDF9
Predicted to enable cytokine activity. Acts upstream of or within female sex determination. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Is expressed in fertilized egg; gonad; and immature gonad. Human ortholog(s) of this gene implicated in primary ovarian insufficiency 14. Orthologous to human GDF9 (growth differentiation factor 9).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
AZQ5MD90 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry, 2-5 μg/ml, Zebrafish
Validation Images & Assay Conditions
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IHC analysis of GDF9 using anti-GDF9 antibody (AZQ5MD90).
GDF9 was detected in a paraffin-embedded section of zebrafish ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GDF9 Antibody (AZQ5MD90) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Zebrafish GDF9 Antibody (AZQ5MD90)
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