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HOOK3 antibody (AZA0A8M6Z0P5) performs well in IHC and shows reactivity with Zebrafish.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IHC analysis of HOOK3 using anti-HOOK3 antibody (AZA0A8M6Z0P5).
HOOK3 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HOOK3 Antibody (AZA0A8M6Z0P5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
HOOK3 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HOOK3 Antibody (AZA0A8M6Z0P5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-Zebrafish HOOK3 Antibody
HOOK3 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Zebrafish | Rabbit | IHC |
Tested Applications
HOOK3 antibody was positively detected by IHC in:
zebrafish brain tissue, zebrafish colon tissue, zebrafish pancreas tissue, zebrafish kidney tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Zebrafish
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Zebrafish HOOK3 Antibody
View all HOOK3 Antibodies
View all HOOK3 Antibodies
Form
Lyophilized
Uniprot
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived zebrafish HOOK3 recombinant protein (Position: E14-R198)
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Background of HOOK3
Protein Hook homolog 3 is a protein that in humans is encoded by the HOOK3 gene. Hook proteins are cytosolic coiled-coil proteins that contain conserved N-terminal domains, which attach to microtubules, and more divergent C-terminal domains, which mediate binding to organelles. The Drosophila Hook protein is a component of the endocytic compartment.
Cite This Product
Anti-Zebrafish HOOK3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZA0A8M6Z0P5)
Protocols
Immunohistochemistry (IHC) protocol for HOOK3 antibody
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Product Promise
We promise Anti-Zebrafish HOOK3 Antibody performs excellently in relevant applications for IHC. If it doesn't perform as promised, we will refund the product. Learn more
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AZA0A8M6Z0P5
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$470.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 9, 2026