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LCP1 antibody (AZQ6P698) performs well in IHC, WB and shows reactivity with Zebrafish.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IHC analysis of LCP1entin/LCP1 using anti-LCP1entin/LCP1 antibody (AZQ6P698).
LCP1entin/LCP1 was detected in a paraffin-embedded section of zebrafish spleen (left) and liver (right) tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LCP1entin/LCP1 Antibody (AZQ6P698) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
LCP1entin/LCP1 was detected in a paraffin-embedded section of zebrafish spleen (left) and liver (right) tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LCP1entin/LCP1 Antibody (AZQ6P698) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-Zebrafish LCP1 Antibody Picoband®
LCP1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Zebrafish | Rabbit | IHC, WB |
Tested Applications
LCP1 antibody was positively detected by WB in:
zebrafish head tissue, whole female zebrafish tissue, whole male zebrafish tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
LCP1 antibody was positively detected by IHC in:
zebrafish spleen (left) and liver (right) tissue, zebrafish brain tissue, zebrafish eye tissue, zebrafish heart tissue, zebrafish kidney tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Zebrafish
Immunohistochemistry
2-5 μg/ml
Zebrafish
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Zebrafish LCP1 Antibody Picoband®
View all LCP1 Antibodies
View all LCP1 Antibodies
Form
Lyophilized
Uniprot
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish LCP1 recombinant protein (Position: M1-E333).
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
WB: zebrafish head tissue, whole female zebrafish tissue, whole male zebrafish tissue
IHC: zebrafish spleen and liver tissue, zebrafish brain tissue, zebrafish eye tissue, zebrafish heart tissue, zebrafish kidney tissue
IHC: zebrafish spleen and liver tissue, zebrafish brain tissue, zebrafish eye tissue, zebrafish heart tissue, zebrafish kidney tissue
Background of LCP1
Predicted to enable actin filament binding activity. Predicted to be involved in actin filament bundle assembly and actin filament network formation. Located in cytoplasm. Is expressed in several structures, including eye; hematopoietic cell; hematopoietic system; mesoderm; and renal system. Human ortholog(s) of this gene implicated in nicotine dependence. Orthologous to human LCP1 (lymphocyte cytosolic protein 1).
Cite This Product
Anti-Zebrafish LCP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZQ6P698)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Zebrafish
100%
High Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for LCP1 antibody
Immunohistochemistry (IHC) protocol for LCP1 antibody
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Product Promise
We promise Anti-Zebrafish LCP1 Antibody Picoband® performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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AZQ6P698
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$470.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 8, 2026