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NAMPT antibody (AZE7F8T6) performs well in IHC and shows reactivity with Zebrafish.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IHC analysis of PBEF/NAMPT using anti-PBEF/NAMPT antibody (AZE7F8T6).
PBEF/NAMPT was detected in a paraffin-embedded section of zebrafish skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PBEF/NAMPT Antibody (AZE7F8T6) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
PBEF/NAMPT was detected in a paraffin-embedded section of zebrafish skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PBEF/NAMPT Antibody (AZE7F8T6) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Designing a Western blot or IHC for NAMPT? The guides cover the expected band size and staining pattern, antibodies backed by real images, the controls to run, and protocols from published papers.
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Anti-Zebrafish PBEF/NAMPT Antibody
NAMPT antibody
| Reactive Species | Host | Application |
|---|---|---|
| Zebrafish | Rabbit | IHC |
Tested Applications
NAMPT antibody was positively detected by IHC in:
zebrafish skeletal muscle tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Zebrafish
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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IHC
IF
Zebrafish
Tested
Recommend
Bovine
Predicted
Predicted
Sheep
Predicted
Predicted
Monkey
Predicted
Predicted
Product Information
Product Name
Anti-Zebrafish PBEF/NAMPT Antibody
View all NAMPT Antibodies
View all NAMPT Antibodies
Form
Lyophilized
Uniprot
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived zebrafish PBEF/NAMPT recombinant protein (Position: G62-N338).
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Background of NAMPT
Nicotinamide phosphoribosyltransferase (NAmPRTase or Nampt), also known as pre-B-cell colony-enhancing factor 1 (PBEF1) or visfatin, is an enzyme that in humans is encoded by the PBEF1 gene. This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging. This gene has a pseudogene on chromosome 10.
Cite This Product
Anti-Zebrafish PBEF/NAMPT Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZE7F8T6)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Zebrafish
100%
High Homology
Cow
90%
High Homology
Sheep
90%
High Homology
Monkey
90%
High Homology
Mouse
89%
Moderate Homology
Rat
89%
Moderate Homology
Human
89%
Moderate Homology
Rabbit
88%
Moderate Homology
Dog
88%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Immunohistochemistry (IHC) protocol for NAMPT antibody
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Product Promise
We promise Anti-Zebrafish PBEF/NAMPT Antibody performs excellently in relevant applications for IHC. If it doesn't perform as promised, we will refund the product. Learn more
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AZE7F8T6
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$470.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 27, 2026