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RAB10 antibody (AZQ6DGV5) performs well in WB and shows reactivity with Zebrafish.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Western blot analysis of RAB10 using anti-RAB10 antibody (AZQ6DGV5).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: whole female zebrafish tissue lysates,
Lane 2: whole male zebrafish tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB10 antigen affinity purified polyclonal antibody (AZQ6DGV5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RAB10 at approximately 22 kDa. The expected band size for RAB10 is at 23 kDa.
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: whole female zebrafish tissue lysates,
Lane 2: whole male zebrafish tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB10 antigen affinity purified polyclonal antibody (AZQ6DGV5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RAB10 at approximately 22 kDa. The expected band size for RAB10 is at 23 kDa.
Designing a Western blot for RAB10? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the RAB10 Western Blot GuideBundle Save
Anti-Zebrafish RAB10 Antibody Picoband®
RAB10 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Zebrafish | Rabbit | WB |
Tested Applications
RAB10 antibody was positively detected by WB in:
whole female zebrafish tissue, whole male zebrafish tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Zebrafish
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Zebrafish RAB10 Antibody Picoband®
View all RAB10 Antibodies
View all RAB10 Antibodies
Form
Lyophilized
Uniprot
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived zebrafish RAB10 recombinant protein (Position: A153-C200).
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
22 kDa
Background of RAB10
Ras-related protein Rab-10 is a protein that in humans is encoded by the RAB10 gene. RAB10 belongs to the RAS superfamily of small GTPases. And RAB proteins localize to exocytic and endocytic compartments and regulate intracellular vesicle trafficking. The RAB10 gene is mapped to chromosome 2p23.1-p22.3 by radiation hybrid analysis. It was found that the purified recombinant GAP domain of human AS160 showed GAP activity with RAB2A, RAB8A, RAB10, and RAB14, but not with 14 other RABs. Immunoblot analysis showed that these RABs associated with Glut4-positive vesicles in mouse adipocytes. Thereby, it concluded that AK160 functions as a RAB GAP and that RABs may participate in GLUT4 translocation.
Cite This Product
Anti-Zebrafish RAB10 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZQ6DGV5)
Protocols
Western Blot protocol for RAB10 antibody
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Product Promise
We promise Anti-Zebrafish RAB10 Antibody Picoband® performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more
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AZQ6DGV5
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$470.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 24, 2026