Anti-Zinc finger protein DPF3 DPF3 Antibody

Product Name

Anti-Zinc finger protein DPF3 DPF3 Antibody

View all DPF3 Antibodies

SKU/Catalog Number

A09485

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Zinc finger protein DPF3 DPF3 Antibody (Catalog # A09485). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

DPF3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-Zinc finger protein DPF3 DPF3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09485)

Host

Rabbit

Contents

DPF3 antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

DPF3 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human DPF3. The immunogen is located within amino acids 220 - 270 of DPF3.

Cross-reactivity

DPF3 antibody is human and mouse reactive. Multiple isoforms of DPF3 are known to exist.

Reactive Species

A09485 is reactive to DPF3 in Human, Mouse

Observed Molecular Weight

68 kDa

Calculated molecular weight

43.1 kDa

Background of DPF3

The DPF3 protein, also known as Cerd4, is a member of the d4 gene family of transcription modulators that also includes DPF1/Neud4 and DPF2/Requiem (1). DPF3 has been shown to be a epigenetic key factor for heart and muscle development and can bind to methylated and acetylated lysine residues of histone 3 and 4, suggesting that DPF3 may play a role in recruiting chromatin remodeling complexes to acetylated histones (2). Two isoforms of DPF3, DPF3a and DPF3b, are required as transcriptional co-activators in SWI/SNF complex-dependent activation of the NF-kappaB RelA/p50 heterodimer (3).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A09485 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

DPF3 antibody can be used for detection of DPF3 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information DPF3

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