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Product Info Summary
| SKU: | A07390 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-Zinc finger protein GLIS2 GLIS2 Antibody
SKU/Catalog Number
A07390
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Zinc finger protein GLIS2 GLIS2 Antibody (Catalog # A07390). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Zinc finger protein GLIS2 GLIS2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07390)
Host
Rabbit
Contents
GLIS2 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
GLIS2 antibody was raised against an 18 amino acid peptide near the center of human GLIS2. The immunogen is located within amino acids 260 - 310 of GLIS2.
Cross-reactivity
GLIS2 antibody is human, mouse and rat reactive.
Reactive Species
A07390 is reactive to GLIS2 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
55.7 kDa
Background of GLIS2
GLIS2, also known as neuronal Krueppel-like, is a 524 amino acid protein that belongs to the GLI C2H2-type zinc-finger protein family (1). GLIS2 can act either as a transcription repressor or as a transcription activator and may be involved in neuron differentiation (1,2). GLIS2 is expressed at high levels in kidney and at low levels in heart, lung and placenta. Mutations of GLIS2 have been suggested to be associated with development of progressive chronic kidney disease with characteristics resembling nephronophthisis (3,4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07390 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
GLIS2 antibody can be used for detection of GLIS2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of GLIS2 in K562 cell lysate with GLIS2 antibody at 1 μg/ml.
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Immunohistochemistry of GLIS2 in mouse kidney tissue with GLIS2 antibody at 5 μg/ml.
Specific Publications For Anti-Zinc finger protein GLIS2 GLIS2 Antibody (A07390)
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