Product Info Summary
SKU: | A17720 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-ZNF287/Zfp287 Antibody
View all Zinc finger protein 287 Antibodies
SKU/Catalog Number
A17720
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-ZNF287/Zfp287 Antibody (Catalog # A17720). Tested in WB, IHC applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ZNF287/Zfp287 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A17720)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human ZNF287.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A17720 is reactive to ZNF287 in Human, Mouse
Applications
A17720 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
88.339kDa
Background of Zinc finger protein 287
May be involved in transcriptional regulation.
Romero-Pastrana F., Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases.
Ebert L., Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from NIH-3T3 cells, using ZNF287 antibody A17720.
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human brain tissue using ZNF287 antibody A17720.
Protein Target Info & Infographic
Gene/Protein Information For ZNF287 (Source: Uniprot.org, NCBI)
Gene Name
ZNF287
Full Name
Zinc finger protein 287
Weight
88.339kDa
Superfamily
krueppel C2H2-type zinc-finger protein family
Alternative Names
MGC15716; Zfp414; zinc finger protein 414 ZNF287 ZKSCAN13, ZSCAN45 zinc finger protein 287 zinc finger protein 287|zinc finger protein with KRAB and SCAN domains 13
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ZNF287, check out the ZNF287 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ZNF287: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ZNF287/Zfp287 Antibody (A17720)
Hello CJ!
No publications found for A17720
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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7 Customer Q&As for Anti-ZNF287/Zfp287 Antibody
Question
I was wanting to use your anti-ZNF287/Zfp287 antibody for WB for human brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brain identification?
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
You can see on the product datasheet, A17720 anti-ZNF287/Zfp287 antibody has been validated for IHC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-03
Question
I have a question about product A17720, anti-ZNF287/Zfp287 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-04
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A17720 anti-ZNF287/Zfp287 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-04
Question
I see that the anti-ZNF287/Zfp287 antibody A17720 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-08-09
Question
Does A17720 anti-ZNF287/Zfp287 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-03
Answer
It shows on the product datasheet, A17720 anti-ZNF287/Zfp287 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-03
Question
I am looking for to test anti-ZNF287/Zfp287 antibody A17720 on human brain for research purposes, then I may be interested in using anti-ZNF287/Zfp287 antibody A17720 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
P. Roberts
Verified customer
Asked: 2018-01-11
Answer
The products we sell, including anti-ZNF287/Zfp287 antibody A17720, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-01-11
Question
Will anti-ZNF287/Zfp287 antibody A17720 work for WB with brain?
B. Singh
Verified customer
Asked: 2016-05-05
Answer
According to the expression profile of brain, ZNF287 is highly expressed in brain. So, it is likely that anti-ZNF287/Zfp287 antibody A17720 will work for WB with brain.
Boster Scientific Support
Answered: 2016-05-05
Question
We are currently using anti-ZNF287/Zfp287 antibody A17720 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on pig tissues as well?
D. Moore
Verified customer
Asked: 2014-12-04
Answer
The anti-ZNF287/Zfp287 antibody (A17720) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-12-04