Boster Bio Life Science Blog

In immunohistochemistry (IHC), using fluorescently labeled antibodies is a common method to detect protein expression and localization. The choice of fluorophores directly affects image quality, signal-to-noise ratio, and experimental reproducibility. Researchers must consider experimental equipment, target abundance, and other conditions to select suitable dyes.

Learn how to analyze ELISA data and calculate results accurately using step-by-step methods. This guide covers standard curve fitting, OD interpretation, and troubleshooting tips to help you improve ELISA assay performance and data reliability.

Flow cytometry is a cornerstone technique for dissecting heterogeneous cell populations based on physical and biochemical properties. Central to its accuracy is gating—a systematic process to isolate target cell subsets while excluding noise from debris, dead cells, or technical artifacts. This guide integrates fundamental principles, step-by-step workflows, and advanced strategies to empower researchers in designing robust gating protocols.

Introduction

In ELISA measurement, there are three

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Western blotting is a powerful technique, but achieving clear and reliable results often depends on addressing common challenges. In this article, we tackle four frequently asked questions to help you optimize your experiments: Why are positive and negative controls essential, and how can you select them? What causes differences between detected and theoretical molecular weight, and how can you resolve these discrepancies? How can you reduce high background in your results? And finally, what leads to multiple non-specific bands, and how can you minimize them? Dive in to find practical tips and solutions for better WB results!

Proper sample processing is crucial to ensuring reliable results in ELISA tests. This blog includes a summary of recommended steps for different sample types, a note on what you must know to collect samples, and a guide on what causes hemolysis in the sample and how to avoid it.

In conducting immunohistochemistry (IHC) and immunofluorescence (IF) experiments, one crucial step is the fixation of cells to preserve them. This prevents cell autolysis and degradation caused by proteolytic enzymes and increases the mechanical strength of the cell structure. Fixation ensures the cell's morphology and structure remain intact, maintaining a "lifelike" appearance.

This article discusses the definition of cell lysis and basic methods, with a focus on the components, selection, and usage steps of chemical lysis buffers.

Cancer metastasis is a leading cause of poor prognosis in cancer patients and represents a central challenge in oncology research. The process of metastasis is highly complex and involves multiple steps: local invasion, entry into the circulatory system, dissemination through blood or lymphatic vessels, seeding in distant tissues, and subsequent growth in a new environment.

Paraformaldehyde is primarily used in biological and biomedical research for fixing cells and tissues. When dissolved in a buffer like PBS (phosphate-buffered saline) to make a 4% solution, its main function is to crosslink proteins within cells and tissues. This fixation process preserves cellular morphology and prevents degradation, enabling various microscopic techniques such as immunostaining and microscopy for detailed analysis of cellular structures and protein localization.