ACOT13 Antibodies

AND ACOT13 ELISA kits, ACOT13 Proteins

Many biological assays use antibodies to detect ACOT13, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with ACOT13 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop ACOT13 antibodies...

We validate the specificity of these antibodies to ACOT13 by testing them on tissues known to express ACOT13 positively and negatively. Browse below to find the ACOT13 antibody that suites your experiment. We have 3 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

Designing an IHC experiment for ACOT13? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.

Open the ACOT13 IHC Guide

ACOT13 Infographic by Boster Bio

All ACOT13 Products

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Specificity

Applications

Price

Anti-THEM2 ACOT13 Antibody

Western blot analysis of THEM2 in HepG2 cell lysate with THEM2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide

Immunofluorescence of THEM2 in human liver tissue with THEM2 antibody at 20 μg/mL

Immunohistochemistry of THEM2 in human liver tissue with THEM2 antibody at 2

Human, Mouse, Rat

ELISA, IF, IHC-P, WB

$481
Cat # A07288-1
0.1 mg

THEM2 (ACOT13) (NM_018473) Human Over-expression Lysate

Human

$628
Cat # LS055856
20 µg, 100 µg

THEM2 (ACOT13) (NM_018473) Human Recombinant Protein

Coomassie blue staining of purified ACOT13 protein

$1249
Cat # PROTQ9NPJ3
20 µg

ACOT13 Overview

Facts about Acyl-coenzyme A thioesterase 13.

ACOT13 3D structure. Source: alphafold

Acyl-coenzyme A thioesterase 13 (ACOT13)

Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs into the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Has acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates.

Can also hydrolyze 3- hydroxyphenylacetyl-CoA and 3,4-dihydroxyphenylacetyl-CoA (in vitro). May play a role in controlling adaptive thermogenesis (By similarity).

Gene Information

Human
Gene Name:	ACOT13
Uniprot:	Q9NPJ3
Entrez:	55856

Family

Belongs to:

thioesterase PaaI family

Alternative Names

acyl-CoA thioesterase 13PNAS-27; EC 3.1.2.-; HT012; hypothalamus protein HT012; MGC4961; THEM2; Thioesterase superfamily member 2acyl-coenzyme A thioesterase 13

Molecular Weight

Mass (kDA):
14.96 kDA

Genomic Context


Human

Location:	6p22.3
Sequence:	6; NC_000006.12 (24667035..24705069)

Subcellular Localizations

Cytoplasm, cytosol. Mitochondrion. Nucleus. Cytoplasm, cytoskeleton, spindle. During interphase, found both in the nucleus and in the cytoplasm. At mitosis, localizes to the spindle. Colocalizes with tubulin.

Diseases

Apraxias
Language Disorders
Impairment (finding)
Steroid Sulfatase Deficiency Disease
Steatosis
Dyslexia

Pathways

Glucose Homeostasis
Oxidative Phosphorylation
Fatty Acid Oxidation

PTMs

Oxidation

Phosphorylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/ACOT13

References

PMID: 16934754 by Cheng Z., et al. Crystal structure of human thioesterase superfamily member 2.

PMID: 19170545 by Cao J., et al. The mechanisms of human hotdog-fold thioesterase 2 (hTHEM2) substrate recognition and catalysis illuminated by a structure and function based analysis.