CHST12 Antibodies

AND CHST12 ELISA kits, CHST12 Proteins

Many biological assays use antibodies to detect CHST12, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with CHST12 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop CHST12 antibodies...

We validate the specificity of these antibodies to CHST12 by testing them on tissues known to express CHST12 positively and negatively. Browse below to find the CHST12 antibody that suites your experiment. We have 3 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

Designing an IHC experiment for CHST12? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.

Open the CHST12 IHC Guide

CHST12 Infographic by Boster Bio

All CHST12 Products

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Product

Figures

Specificity

Applications

Price

Anti-CHST12 Antibody (N-term)

Western blot analysis of lysate from human spleen tissue lysate, using CHST12 Antibody (N-term)

Anti-CHST12 Antibody (N-term) at 1:2000 dilution + HL-60 whole cell lysatesLysates/proteins at 20 µg per lane

Human

IHC-P, WB

$299
Cat # A10456-1
80 µl

CHST12 (NM_018641) Human Over-expression Lysate

Human

$628
Cat # LS055501
20 µg, 100 µg

CHST12 (NM_018641) Human Recombinant Protein

Coomassie blue staining of purified CHST12 protein

$1249
Cat # PROTQ9NRB3
20 µg

CHST12 Overview

Facts about Carbohydrate sulfotransferase 12.

CHST12 3D structure. Source: alphafold

Carbohydrate sulfotransferase 12 (CHST12)

Catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin and desulfated dermatan sulfate. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of cells and extracellular matrices.

Activity toward partially desulfated dermatan sulfate is however lower. Doesn't form 4, 6-di-O-sulfated GalNAc if chondroitin sulfate C is used as an acceptor.

Gene Information

Human
Gene Name:	CHST12
Uniprot:	Q9NRB3
Entrez:	55501

Family

Belongs to:

sulfotransferase 2 family

Alternative Names

C4S-2; C4ST2C4ST-2; carbohydrate (chondroitin 4) sulfotransferase 12; carbohydrate sulfotransferase 12; Chondroitin 4-O-sulfotransferase 2; Chondroitin 4-sulfotransferase 2; EC 2.8.2.5; Sulfotransferase Hlo

Molecular Weight

Mass (kDA):
48.414 kDA

Genomic Context


Human

Location:	7p22.3
Sequence:	7; NC_000007.14 (2403489..2448484)

Tissue Specificity

Widely expressed. Expressed a high level in spinal chord, heart, spleen, thyroid, pituitary gland, adrenal gland, peripheral blood leukocytes, thymus, lung, small intestine, fetal kidney, fetal spleen and fetal lung.

Subcellular Localizations

Golgi apparatus membrane; Single-pass type II membrane protein.

Diseases

Sclerosis
Multiple Sclerosis

Pathways

Sulfation
Cellular Process

PTMs

Glycoprotein

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/CHST12

References

PMID: 10781601 by Hiraoka N., et al. Molecular cloning and expression of two distinct human chondroitin 4- O-sulfotransferases that belong to the HNK-1 sulfotransferase gene family.

PMID: 12847091 by Mikami T., et al. Specificities of three distinct human chondroitin/dermatan N- acetylgalactosamine 4-O-sulfotransferases demonstrated using partially desulfated dermatan sulfate as an acceptor. Implication of differential roles in dermatan sulfate biosynthesis.